BRENDA - Enzyme Database

Gene cloning and characterization of CDP-diacylglycerol synthase from rat brain

Saito, S.; Goto, K.; Tonosaki, A.; Kondo, H.; J. Biol. Chem. 272, 9503-9509 (1997)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.7.41
-
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
phosphatidylinositol 4,5-bisphosphate
strong
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
0.102
-
1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphate
pH 7.5, 30°C
Rattus norvegicus
2.7.7.41
0.114
-
1,2-dioleoyl-sn-glycero-3-phosphate
pH 7.5, 30°C
Rattus norvegicus
2.7.7.41
0.138
-
phosphatidic acid
pH 7.5, 30°C
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
membrane
the enzyme is mainly localized in close association with the membrane of the endoplasmic reticulum of the transfected cells. Several possible membrane-spanning regions
Rattus norvegicus
16020
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.41
Rattus norvegicus
O35052
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.7.41
brain
-
Rattus norvegicus
-
2.7.7.41
cerebellar Purkinje cell
-
Rattus norvegicus
-
2.7.7.41
photoreceptor
inner segment of
Rattus norvegicus
-
2.7.7.41
pineal gland
-
Rattus norvegicus
-
2.7.7.41
spermatid
-
Rattus norvegicus
-
2.7.7.41
spermatocyte
postmitotic
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + 1-stearoyl-2-arachidonoyl phosphatidic acid
-
643325
Rattus norvegicus
diphosphate + CDP-1-stearoyl-2-arachidonoylglycerol
-
-
-
?
2.7.7.41
CTP + phosphatidate
the enzyme prefers 1-stearoyl-2-arachidonoyl phosphatidic acid as substrate. Little or no activity is detected towards phosphatidic acids containing saturated fatty acyl groups in both the sn-1 and sn-2 positions
643325
Rattus norvegicus
diphosphate + CDPdiacylglycerol
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.41
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
phosphatidylinositol 4,5-bisphosphate
strong
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
0.102
-
1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphate
pH 7.5, 30°C
Rattus norvegicus
2.7.7.41
0.114
-
1,2-dioleoyl-sn-glycero-3-phosphate
pH 7.5, 30°C
Rattus norvegicus
2.7.7.41
0.138
-
phosphatidic acid
pH 7.5, 30°C
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
membrane
the enzyme is mainly localized in close association with the membrane of the endoplasmic reticulum of the transfected cells. Several possible membrane-spanning regions
Rattus norvegicus
16020
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.7.41
brain
-
Rattus norvegicus
-
2.7.7.41
cerebellar Purkinje cell
-
Rattus norvegicus
-
2.7.7.41
photoreceptor
inner segment of
Rattus norvegicus
-
2.7.7.41
pineal gland
-
Rattus norvegicus
-
2.7.7.41
spermatid
-
Rattus norvegicus
-
2.7.7.41
spermatocyte
postmitotic
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + 1-stearoyl-2-arachidonoyl phosphatidic acid
-
643325
Rattus norvegicus
diphosphate + CDP-1-stearoyl-2-arachidonoylglycerol
-
-
-
?
2.7.7.41
CTP + phosphatidate
the enzyme prefers 1-stearoyl-2-arachidonoyl phosphatidic acid as substrate. Little or no activity is detected towards phosphatidic acids containing saturated fatty acyl groups in both the sn-1 and sn-2 positions
643325
Rattus norvegicus
diphosphate + CDPdiacylglycerol
-
-
-
?