BRENDA - Enzyme Database

CTP-phosphatidic acid cytidyltransferase from Saccharomyces cerevisiae. Partial purification, characterization, and kinetic behavior

Belendiuk, G.; Mangnall, D.; Tung, B.; Westley, J.; Getz, G.S.; J. Biol. Chem. 253, 4555-4565 (1978)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.7.7.41
the solubilized protein is quite labile. Its stability is markedly enhanced by exchange dialysis against 10% glycerol, 20% propylene glycol, 25 mM cacodylate buffer, pH 6.5, 10 mM MgCl2
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
dCTP
2.5 mM, 54% inhibition of purified enzyme; competitive with respect to CTP
Saccharomyces cerevisiae
2.7.7.41
diphosphate
2.5 mM, 80% inhibition of purified enzyme, noncompetitive with respect to CTP and phosphatidic acid
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
mitochondrial membrane
-
Saccharomyces cerevisiae
31966
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.41
400000
-
approximately, gel filtration
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.41
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.7.41
partial
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.41
0.45
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + phosphatidate
-
643310
Saccharomyces cerevisiae
diphosphate + CDPdiacylglycerol
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
2.7.7.41
More
two distinct protein bands of 45000 Da and 19000 Da are detected by SDS-PAGE, it is yet unclear whether this represents simple aggregation of two unrelated proteins in a monomer dimer relationship, or whether the enzyme has two distinct subunits present in a 1:4 molar ratio
Saccharomyces cerevisiae
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.7.41
the solubilized protein is quite labile. Its stability is markedly enhanced by exchange dialysis against 10% glycerol, 20% propylene glycol, 25 mM cacodylate buffer, pH 6.5, 10 mM MgCl2
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
dCTP
2.5 mM, 54% inhibition of purified enzyme; competitive with respect to CTP
Saccharomyces cerevisiae
2.7.7.41
diphosphate
2.5 mM, 80% inhibition of purified enzyme, noncompetitive with respect to CTP and phosphatidic acid
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
mitochondrial membrane
-
Saccharomyces cerevisiae
31966
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.41
400000
-
approximately, gel filtration
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.41
partial
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.41
0.45
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + phosphatidate
-
643310
Saccharomyces cerevisiae
diphosphate + CDPdiacylglycerol
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.7.41
More
two distinct protein bands of 45000 Da and 19000 Da are detected by SDS-PAGE, it is yet unclear whether this represents simple aggregation of two unrelated proteins in a monomer dimer relationship, or whether the enzyme has two distinct subunits present in a 1:4 molar ratio
Saccharomyces cerevisiae