BRENDA - Enzyme Database

Purification and properties of the membrane-bound CDP-diglyceride synthetase from Escherichia coli

Sparrow, C.P.; Raetz, C.R.H.; J. Biol. Chem. 260, 12084-12091 (1985)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
CTP
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
0.28
-
phosphatidic acid
pH 7.4, 30░C
Escherichia coli
2.7.7.41
0.58
-
dCTP
pH 7.4, 30░C
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.41
Escherichia coli
-
K12 strain DH1 with plasmid pCD100 causing 53fold overproduction of the enzyme
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.7.41
-
Escherichia coli
Reaction
EC Number
Reaction
Commentary
Organism
2.7.7.41
CTP + phosphatidate = diphosphate + CDP-diacylglycerol
sequential mechanism
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.7.41
30.79
-
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + 1,2-dioleoyl phosphatidic acid
reaction with 1-oleoyl-2-palmitoyl-phosphatidic acid at 87.5% of the activity with phosphatidic acid from egg phosphatidylcholine
643308
Escherichia coli
diphosphate + CDP-dioleoylglycerol
-
-
-
-
2.7.7.41
CTP + 1,2-dipalmitoyl phosphatidic acid
reaction with 1-oleoyl-2-palmitoyl phosphatidic acid at 4.6% of the activity with phosphatidic acid from egg phosphatidylcholine
643308
Escherichia coli
diphosphate + CDP-dipalmitoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-oleoyl-2-palmitoyl phosphatidic acid
reaction with 1-oleoyl-2-palmitoyl phosphatidic acid at 83.4% of the activity with phosphatidic acid from egg phosphatidylcholine
643308
Escherichia coli
diphosphate + CDP-1-oleoyl-2-palmitoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-palmitoyl-2-oleoyl phosphatidic acid
reaction with 1-oleoyl-2-palmitoyl phosphatidic acid at 99.7% of the activity with phosphatidic acid from egg phosphatidylcholine
643308
Escherichia coli
diphosphate + CDP-1-palmitoyl-2-oleoylglycerol
-
-
-
?
2.7.7.41
CTP + phosphatidate
high activity with phosphatidic acid from egg phosphatidylcholine
643308
Escherichia coli
diphosphate + CDPdiacylglycerol
-
-
-
r
2.7.7.41
CTP + phosphatidate
equilibrium constant is 0.22
643308
Escherichia coli
diphosphate + CDPdiacylglycerol
-
-
-
r
2.7.7.41
dCTP + phosphatidate
reaction at the same rate as CTP
643308
Escherichia coli
diphosphate + dCDPdiacylglycerol
-
-
-
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.7.41
0.59
-
CTP
pH 7.4, 30░C
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
CTP
-
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.7.41
0.59
-
CTP
pH 7.4, 30░C
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
0.28
-
phosphatidic acid
pH 7.4, 30░C
Escherichia coli
2.7.7.41
0.58
-
dCTP
pH 7.4, 30░C
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.41
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.7.41
30.79
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + 1,2-dioleoyl phosphatidic acid
reaction with 1-oleoyl-2-palmitoyl-phosphatidic acid at 87.5% of the activity with phosphatidic acid from egg phosphatidylcholine
643308
Escherichia coli
diphosphate + CDP-dioleoylglycerol
-
-
-
-
2.7.7.41
CTP + 1,2-dipalmitoyl phosphatidic acid
reaction with 1-oleoyl-2-palmitoyl phosphatidic acid at 4.6% of the activity with phosphatidic acid from egg phosphatidylcholine
643308
Escherichia coli
diphosphate + CDP-dipalmitoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-oleoyl-2-palmitoyl phosphatidic acid
reaction with 1-oleoyl-2-palmitoyl phosphatidic acid at 83.4% of the activity with phosphatidic acid from egg phosphatidylcholine
643308
Escherichia coli
diphosphate + CDP-1-oleoyl-2-palmitoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-palmitoyl-2-oleoyl phosphatidic acid
reaction with 1-oleoyl-2-palmitoyl phosphatidic acid at 99.7% of the activity with phosphatidic acid from egg phosphatidylcholine
643308
Escherichia coli
diphosphate + CDP-1-palmitoyl-2-oleoylglycerol
-
-
-
?
2.7.7.41
CTP + phosphatidate
high activity with phosphatidic acid from egg phosphatidylcholine
643308
Escherichia coli
diphosphate + CDPdiacylglycerol
-
-
-
r
2.7.7.41
CTP + phosphatidate
equilibrium constant is 0.22
643308
Escherichia coli
diphosphate + CDPdiacylglycerol
-
-
-
r
2.7.7.41
dCTP + phosphatidate
reaction at the same rate as CTP
643308
Escherichia coli
diphosphate + dCDPdiacylglycerol
-
-
-
?