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Literature summary extracted from

  • Thorson, J.S.; Kelly, T.M.; Liu, H.W.
    Cloning, sequencing, and overexpression in Escherichia coli of the alpha-D-glucose-1-phosphate cytidylyltransferase gene isolated from Yersinia pseudotuberculosis (1994), J. Bacteriol., 176, 1840-1849.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.33
-
 Yersinia pseudotuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.33 0.079
-
 alpha-D-glucose 1-phosphate pH 7.5, 37°C Yersinia pseudotuberculosis
2.7.7.33 0.082
-
 CTP pH 7.5, 37°C Yersinia pseudotuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.33 Co2+ with decreasing order of efficiency: Co2+, Mn2+, Mg2+, mixture of Mn2+ and Mg2+ Yersinia pseudotuberculosis
2.7.7.33 Mg2+ with decreasing order of efficiency: Co2+, Mn2+, Mg2+, mixture of Mn2+ and Mg2+ Yersinia pseudotuberculosis
2.7.7.33 Mn2+ with decreasing order of efficiency: Co2+, Mn2+, Mg2+, mixture of Mn2+ and Mg2+ Yersinia pseudotuberculosis
2.7.7.33 additional information no activation by Ni2+ Yersinia pseudotuberculosis
2.7.7.33 additional information no activation by Cu2+ Yersinia pseudotuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.33 29000
-
 4 * 29000, SDS-PAGE Yersinia pseudotuberculosis
2.7.7.33 116000
-
 gel filtration Yersinia pseudotuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.33 CTP + D-glucose 1-phosphate Yersinia pseudotuberculosis first step in biosynthesis of CDP-ascarylose ?
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.33 Yersinia pseudotuberculosis Q57430
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.33 2.51
-
-
 Yersinia pseudotuberculosis

Storage Stability

EC Number Storage Stability Organism
2.7.7.33 -80°C stable for months with no loss of activity, freezing and thawing does not affect the enzyme activity Yersinia pseudotuberculosis
2.7.7.33 4°C stable for days Yersinia pseudotuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.33 CTP + alpha-D-glucose 1-phosphate
-
 Yersinia pseudotuberculosis diphosphate + CDPglucose
-
 r
2.7.7.33 CTP + D-glucose 1-phosphate first step in biosynthesis of CDP-ascarylose Yersinia pseudotuberculosis ?
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.33 tetramer 4 * 29000, SDS-PAGE Yersinia pseudotuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.33 37
-
 assay at Yersinia pseudotuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.33 7 9
-
 Yersinia pseudotuberculosis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.33 6
-
 irreversible inactivation below pH 6 Yersinia pseudotuberculosis