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Literature summary extracted from

  • Mayer, R.M.; Ginsburg, V.
    Purification and properties of cytidine diphosphate D-glucose pyrophosphorylase from Salmonella paratyphi A (1965), J. Biol. Chem., 240, 1900-1904.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.33 CDPparatose synonym: 3,6-dideoxy-D-glucose, feed-back inhibition, CDPglucose partially reverses, maximal inhibition at 0.6 mM Salmonella enterica subsp. enterica serovar Paratyphi A
2.7.7.33 additional information little or no inhibition by CMP, GDPglucose, UDPglucose, dTDPglucose; no inhibition by NaF, diphosphatase, 2-mercaptoethanol, paratose, CTP Salmonella enterica subsp. enterica serovar Paratyphi A

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.33 0.015
-
 CDPglucose pH 8, 37°C Salmonella enterica subsp. enterica serovar Paratyphi A
2.7.7.33 0.034
-
 CTP pH 8, 37°C Salmonella enterica subsp. enterica serovar Paratyphi A
2.7.7.33 0.09
-
 alpha-D-glucose 1-phosphate pH 8, 37°C Salmonella enterica subsp. enterica serovar Paratyphi A
2.7.7.33 0.1
-
 diphosphate pH 8, 37°C Salmonella enterica subsp. enterica serovar Paratyphi A

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.33 Mg2+ requirement Salmonella enterica subsp. enterica serovar Paratyphi A
2.7.7.33 Mg2+ optimal concentration: 3 mM MgCl2 Salmonella enterica subsp. enterica serovar Paratyphi A

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.33 CTP + D-glucose 1-phosphate Salmonella enterica subsp. enterica serovar Paratyphi A branch-point in glucose 1-phosphate anabolism ?
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.33 Salmonella enterica subsp. enterica serovar Paratyphi A
-
 strain a
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.33 0.408
-
-
 Salmonella enterica subsp. enterica serovar Paratyphi A

Storage Stability

EC Number Storage Stability Organism
2.7.7.33 -20°C, about 30% loss of activity within 1 month Salmonella enterica subsp. enterica serovar Paratyphi A

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrates in the reverse reaction: ADPglucose, dTDPglucose, UDPglucose Salmonella enterica subsp. enterica serovar Paratyphi A diphosphate + CDPglucose
-
 r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrate in the reverse reaction: CDPparatose Salmonella enterica subsp. enterica serovar Paratyphi A diphosphate + CDPglucose
-
 r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrate in the forward reaction: alpha-D-mannose 1-phosphate, N-acetyl-alpha-D-glucosamine 1-phosphate Salmonella enterica subsp. enterica serovar Paratyphi A diphosphate + CDPglucose
-
 r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate specific for base and sugar Salmonella enterica subsp. enterica serovar Paratyphi A diphosphate + CDPglucose
-
 r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrate in the forward reaction: GTP and D-mannose 1-phosphate Salmonella enterica subsp. enterica serovar Paratyphi A diphosphate + CDPglucose
-
 r
2.7.7.33 CTP + alpha-D-glucose 1-phosphate no substrates in the reverse reaction: GDPglucose Salmonella enterica subsp. enterica serovar Paratyphi A diphosphate + CDPglucose
-
 r
2.7.7.33 CTP + D-glucose 1-phosphate branch-point in glucose 1-phosphate anabolism Salmonella enterica subsp. enterica serovar Paratyphi A ?
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.33 37
-
 assay at Salmonella enterica subsp. enterica serovar Paratyphi A

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.33 8
-
-
 Salmonella enterica subsp. enterica serovar Paratyphi A

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.33 6.2 9.5 about half-maximal activity at pH 6.2 and about 70% of maximal activity at pH 9.5 Salmonella enterica subsp. enterica serovar Paratyphi A