Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Grosse, F.; Manns, A.
    Terminal deoxyribonucleotidyl transferase (1993), Methods Mol. Biol., 16, 95-105.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.7.31 biotechnology use in production of synthetic homo- and heteropolymers, N-acetylation or N-alkylation of derivatives of dNTPs Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.31
-
 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.31 Cl-
-
 Homo sapiens
2.7.7.31 I-
-
 Homo sapiens
2.7.7.31 NH4+
-
 Homo sapiens
2.7.7.31 phosphate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.31 0.001
-
 oligonucleotide primers
-
 Homo sapiens
2.7.7.31 0.01
-
 dATP dGTP Homo sapiens
2.7.7.31 0.5
-
 dTTP dCTP Homo sapiens
2.7.7.31 1
-
 homopolymer primers
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.31 Co2+ decreasing order of efficiency for elongation of oligonucleotide primers with dATP: Mg2+, Zn2+, Co2+ Homo sapiens
2.7.7.31 Mg2+ decreasing order of efficiency for elongation of oligonucleotide primers with dATP: Mg2+, Zn2+, Co2+ Homo sapiens
2.7.7.31 Mg2+ dGTP also optimally added in the presence of Mg2+ Homo sapiens
2.7.7.31 Mg2+ polymerization of pyrimidines is best with Co2+ Homo sapiens
2.7.7.31 Zn2+ in decreasing order of efficiency for elongation of oligonucleotide primers with dATP: Mg2+, Zn2+, Co2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.31 60000
-
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.31 deoxynucleoside triphosphate + DNA Homo sapiens involved in creating somatic diversity of immunoglobulin and T-cell receptor genes diphosphate + DNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.31 Homo sapiens
-
 commercial preparation
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.31 lymphocyte enzyme activity is only found in the nuclei of pre-T and pre-B lymphocytes Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.31 dATP + DNAn
-
 Homo sapiens diphosphate + DNAn+1
-
 ?
2.7.7.31 deoxynucleoside triphosphate + DNA involved in creating somatic diversity of immunoglobulin and T-cell receptor genes Homo sapiens diphosphate + DNAn+1
-
 ?
2.7.7.31 deoxynucleoside triphosphate + DNAn primer required Homo sapiens diphosphate + DNAn+1
-
 ?
2.7.7.31 deoxynucleoside triphosphate + DNAn reaction is not template directed Homo sapiens diphosphate + DNAn+1
-
 ?
2.7.7.31 dNTP + homopolymer primers
-
 Homo sapiens ?
-
 ?
2.7.7.31 dNTP + oligonucleotide primers
-
 Homo sapiens ?
-
 ?
2.7.7.31 dTTP + DNAn
-
 Homo sapiens diphosphate + DNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.31 monomer 1 * 60000, various organism Homo sapiens