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Literature summary extracted from

  • Gomez-Casati, D.F.; Iglesias, A.A.
    ADP-glucose pyrophosphorylase from wheat endosperm. Purification and characterization of an enzyme with novel regulatory properties (2002), Planta, 214, 428-434.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate activation of leaf enzyme, however endosperm enzyme only, when phosphate, ADP or fructose 1,6-bisphosphate are present Triticum aestivum
2.7.7.27 D-fructose 1,6-bisphosphate activation of leaf enzyme, inhibition of endosperm enzyme Triticum aestivum
2.7.7.27 D-fructose 6-phosphate activation of leaf enzyme, no influence on endosperm enzyme Triticum aestivum
2.7.7.27 phosphoenolpyruvate
-
 Triticum aestivum

General Stability

EC Number General Stability Organism
2.7.7.27 endosperm enzyme stability is increased by ammonium sulfate, as a supernatant of 30% saturing retains 50% of the activity after 5 min heating at 60°C, by additionally polyethylenegycol retains 70% of enzyme activity Triticum aestivum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 ADP inhibition of endosperm enzyme, not leaf enzyme Triticum aestivum
2.7.7.27 D-fructose 6-phosphate only endosperm enzyme is inhibited, activation of leaf enzyme Triticum aestivum
2.7.7.27 phosphate phosphate inhibits leaf and endosperm enzymes, 3-phosphoglycerate and D-fructose 6-phosphate reverse endosperm inhibition Triticum aestivum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.27 chloroplast
-
 Triticum aestivum 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.27 51000
-
 leaf: x * 51000 + x * 54000, endosperm: x * 52000 + x * 53000, SDS-PAGE Triticum aestivum
2.7.7.27 52000
-
 leaf: x * 51000 + x * 54000, endosperm: x * 52000 + x * 53000, SDS-PAGE Triticum aestivum
2.7.7.27 53000
-
 leaf: x * 51000 + x * 54000, endosperm: x * 52000 + x * 53000, SDS-PAGE Triticum aestivum
2.7.7.27 54000
-
 leaf: x * 51000 + x * 54000, endosperm: x * 52000 + x * 53000, SDS-PAGE Triticum aestivum
2.7.7.27 210000
-
 endosperm enzyme Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Triticum aestivum P12299 wheat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27
-
 Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.27 endosperm
-
 Triticum aestivum
-
2.7.7.27 leaf
-
 Triticum aestivum
-
2.7.7.27 seed
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 additional information
-
 the amount of enzyme activity is higher in endosperm than in leaves fresh tissue Triticum aestivum
2.7.7.27 2.4
-
 endosperm enzyme Triticum aestivum
2.7.7.27 58.5
-
 leaf enzyme Triticum aestivum

Storage Stability

EC Number Storage Stability Organism
2.7.7.27 -20°C, purified endosperm enzyme, 20% loss of activity after 1 month Triticum aestivum
2.7.7.27 -20°C, purified leaf enzyme, stable for at least 3 months Triticum aestivum
2.7.7.27 4°C, endosperm extract, 6-8 h, 50% loss of activity Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Triticum aestivum diphosphate + ADP-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.27 heterotetramer leaf: x * 51000 + x * 54000, endosperm: x * 52000 + x * 53000, SDS-PAGE Triticum aestivum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.27 60
-
 leaf enzyme remains stable after 5 min heating at 60°C, the heat treatment of endosperm enzyme results in 95% loss of activity Triticum aestivum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.27 0.025
-
 phosphate pH 8.0, in absence of 0.25 mM 3-phosphoglycerate Triticum aestivum
2.7.7.27 0.2
-
 phosphate leaf enzyme in absence of 3-phosphoglycerate Triticum aestivum
2.7.7.27 0.7
-
 phosphate endosperm enzyme in absence of 3-phosphoglycerate Triticum aestivum
2.7.7.27 0.91
-
 phosphate endosperm enzyme in presence of 0.25 mM 3-phosphoglycerate Triticum aestivum
2.7.7.27 1.52
-
 phosphate endosperm enzyme in presence of 1 mM 3-phosphoglycerate Triticum aestivum
2.7.7.27 2.49
-
 phosphate endosperm enzyme in presence of 5 mM 3-phosphoglycerate Triticum aestivum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.27 Triticum aestivum endosperm enzyme, isoelectric focusing
-
 6.1