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Literature summary extracted from

  • Gomez-Casati, D.F.; Preiss, J.; Iglesias, A.A.
    Studies on the effect of temperature on the activity and stability of cyanobacterial ADP-glucose pyrophosphorylase (2000), Arch. Biochem. Biophys., 384, 319-326.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate kinetic study at different temperatures Anabaena sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 phosphate effect of temperature on the activity of enzyme in the presence of phosphate Anabaena sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 0.11
-
 ATP pH 7.5, 5°C, kinetic study Anabaena sp.
2.7.7.27 0.17
-
 alpha-D-glucose 1-phosphate pH 7.5, 30°C, kinetic study Anabaena sp.
2.7.7.27 0.55
-
 alpha-D-glucose 1-phosphate pH 7.5, 5°C, kinetic study Anabaena sp.
2.7.7.27 0.61
-
 ATP pH 7.5, 30°C, kinetic study Anabaena sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.27 Mg2+
-
 Anabaena sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Anabaena sp. one of the main regulatory steps in starch biosynthesis in plants diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Anabaena sp. major regulated step in the bacterial glycogen biosynthesis pathway diphosphate + ADP-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Anabaena sp.
-
 PCC 7120, cyanobacterium
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Anabaena sp. diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate one of the main regulatory steps in starch biosynthesis in plants Anabaena sp. diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate major regulated step in the bacterial glycogen biosynthesis pathway Anabaena sp. diphosphate + ADP-glucose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.27 37 40 in absence of phosphate Anabaena sp.
2.7.7.27 42 45 in presence of phosphate or in presence of 3-phosphoglycerate Anabaena sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.27 additional information
-
 the Arrhenius plots of the data of enzyme assayed in the temperature range 0-40°C exhibit a transition temperature in the range 9-12°C and suggest that the enzyme undergoes conformational changes at different temperatures Anabaena sp.
2.7.7.27 40
-
 the enzyme is unstable at temperatures higher than 40°C in the absence of allosteric activators Anabaena sp.
2.7.7.27 48
-
 half-life: 68 s, irreversible thermal inactivation at 48°C and above, inactivation is temperature- and time-dependendent Anabaena sp.
2.7.7.27 54
-
 half-life: 41 s Anabaena sp.
2.7.7.27 60
-
 half-life: 25 s, comparison of effect of different metabolites on the thermal inactivation of enzyme Anabaena sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.27 0.18
-
 phosphate pH 7.5, 30°C, in the absence of 3-phosphoglycerate Anabaena sp.
2.7.7.27 1.5
-
 phosphate pH 7.5, 5°C, in the absence of 3-phosphoglycerate Anabaena sp.