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Literature summary extracted from

  • Wang-Gillam, A.; Pastuszak, I.; Stewart, M.; Drake, R.R.; Elbein, A.D.
    Identification and modification of the uridine-binding site of the UDP-GalNAc (GlcNAc) pyrophosphorylase (2000), J. Biol. Chem., 275, 1433-1438.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.23 AGX1 expressed in Escherichia coli as GST fusion protein Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.23 G111A very low activity Homo sapiens
2.7.7.23 G222A traces of activity in forward and reverse reaction with N-acetyl-D-glucosamine 1-phosphate and N-acetyl-D-galactosamine 1-phosphate Homo sapiens
2.7.7.23 G224A low activity in forward and reverse reaction with N-acetyl-D-glucosamine 1-phosphate and N-acetyl-D-galactosamine 1-phosphate Homo sapiens
2.7.7.23 P220A only slight changes in activity with N-acetyl-D-glucosamine 1-phosphate and N-acetyl-D-galactosamine 1-phosphate Homo sapiens
2.7.7.23 R115A slight changes in Km Homo sapiens
2.7.7.23 Y227A only slight changes in activity with N-acetyl-D-glucosamine 1-phosphate and N-acetyl-D-galactosamine 1-phosphate Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.23 0.7
-
 UDP-N-acetyl-D-glucosamine AGX1, pH 8.5, 37°C Homo sapiens
2.7.7.23 0.8
-
 UDP-N-acetyl-D-glucosamine P220A, Y227A, R115A mutants, pH 8.5, 37°C Homo sapiens
2.7.7.23 2.2
-
 UDP-N-acetyl-D-glucosamine G224A mutants, pH 8.5, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine Homo sapiens amino sugar metabolism UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine Homo sapiens involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.23 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.23 GST fusion protein from E. coli Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine
-
 Homo sapiens UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine amino sugar metabolism Homo sapiens UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin Homo sapiens UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.7.23 AGX1
-
 Homo sapiens