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Literature summary extracted from

  • Das Gupta, J.; Li, Q.; Thomson, A.B.; Hunt, A.G.
    Characterization of a novel plant poly(A) polymerase (1995), Plant Sci., 110, 215-226.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.19 3'-dATP
-
 Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.19 0.039
-
 ATP 30°C, pH 8.0 Pisum sativum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.19 Mg2+
-
 Zea mays
2.7.7.19 Mg2+ more active in presence of Mg2+ than Mn2+ Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.19 Brassica juncea
-
-
-
2.7.7.19 Pisum sativum
-
-
-
2.7.7.19 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.19
-
 Pisum sativum
2.7.7.19
-
 Zea mays
2.7.7.19
-
 Brassica juncea

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.7.7.19 chromatography on MonoQ results in three fractions with little activity, one of them containing a 43000 polypeptide, the others RNAs, mixtures of them result in substantial enzymic activity Pisum sativum
2.7.7.19 chromatography on MonoQ results in three fractions with little activity, one of them containing a 43000 polypeptide, the others RNAs, mixtures of them result in substantial enzymic activity Zea mays
2.7.7.19 chromatography on MonoQ results in three fractions with little activity, one of them containing a 43000 polypeptide, the others RNAs, mixtures of them result in substantial enzymic activity Brassica juncea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.19 ATP + RNA
-
 Pisum sativum diphosphate + RNA(A)n
-
 ?
2.7.7.19 ATP + RNA
-
 Zea mays diphosphate + RNA(A)n
-
 ?
2.7.7.19 ATP + RNA
-
 Brassica juncea diphosphate + RNA(A)n
-
 ?