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Literature summary extracted from

  • Lingner, J.; Radtke, I.; Wahle, E.; Keller, W.
    Purification and characterization of poly(A) polymerase from Saccharomyces cerevisiae (1991), J. Biol. Chem., 266, 8741-8746.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.19 N-ethylmaleimide
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.19 additional information
-
 additional information dependence on divalent cation concentration Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.19 KCl optimal concentration: 60 mM Saccharomyces cerevisiae
2.7.7.19 Mg2+ more active in presence of Mn2+ than Mg2+ Saccharomyces cerevisiae
2.7.7.19 Mg2+ optimal concentration depends on ATP concentration Saccharomyces cerevisiae
2.7.7.19 Mn2+ more active in presence of Mn2+ than Mg2+ Saccharomyces cerevisiae
2.7.7.19 Mn2+ optimal concentration depends on ATP concentration Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.19 47000
-
 gel filtration Saccharomyces cerevisiae
2.7.7.19 63000
-
 1 * 63000, SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.19 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.19
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.19 ATP + RNA highly specific for ATP Saccharomyces cerevisiae diphosphate + RNA(A)n no apparent length limitation for the poly(A) tail synthesized ?
2.7.7.19 ATP + RNA primer: various E. coli tRNAs or rRNAs Saccharomyces cerevisiae diphosphate + RNA(A)n no apparent length limitation for the poly(A) tail synthesized ?
2.7.7.19 ATP + RNA elongation of the primer is distributive Saccharomyces cerevisiae diphosphate + RNA(A)n no apparent length limitation for the poly(A) tail synthesized ?
2.7.7.19 ATP + RNA primer required Saccharomyces cerevisiae diphosphate + RNA(A)n no apparent length limitation for the poly(A) tail synthesized ?
2.7.7.19 ATP + RNA primer: RNA homopolymers Saccharomyces cerevisiae diphosphate + RNA(A)n no apparent length limitation for the poly(A) tail synthesized ?

Subunits

EC Number Subunits Comment Organism
2.7.7.19 monomer 1 * 63000, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.19 7
-
 Mn2+-activated enzyme Saccharomyces cerevisiae