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Literature summary extracted from

  • Winters, M.A.; Edmonds, M.
    A poly(A) polymerase from calf thymus. Purification and properities of the enzyme (1973), J. Biol. Chem., 248, 4756-4762.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.19 cordycepin not Bos taurus
2.7.7.19 Ionic strength
-
 Bos taurus
2.7.7.19 K+ 100 mM; KCl Bos taurus
2.7.7.19 additional information not inhibitory: actinomycin D; not inhibitory: alpha-amanitin Bos taurus
2.7.7.19 NH4+ 10-40 mM; ammonium sulfate Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.19 nucleus
-
 Bos taurus 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.19 Mg2+ more active in presence of Mg2+ than Mn2+ Bos taurus
2.7.7.19 Mn2+ more active in presence of Mg2+ than Mn2+ Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.19 140000 160000 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.19 Bos taurus
-
 calf
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.19 calf Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.19 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.19 additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.19 ATP + RNA highly specific for ATP Bos taurus diphosphate + RNA(A)n
-
 ?
2.7.7.19 ATP + RNA primer required Bos taurus diphosphate + RNA(A)n
-
 ?
2.7.7.19 ATP + RNA no specificity for the 3'-terminal nucleotides Bos taurus diphosphate + RNA(A)n
-
 ?
2.7.7.19 ATP + RNA rather low specificity for primer Bos taurus diphosphate + RNA(A)n
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.19 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.19 8.2
-
-
 Bos taurus