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Literature summary extracted from

  • Wang, X.; Moore, T.S.
    Phosphatidylylcholine biosynthesis in castor bean endosperm. Purification and properties of cytidine 5'-triphosphate:choline-phosphate cytidylyltransferase (1990), Plant Physiol., 93, 250-255.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.7.7.15 Unstable at low protein concentration Ricinus communis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.15 ATP inhibition after preincubation with 3 mM ATP for 20 min Ricinus communis
2.7.7.15 additional information no inhibition by ATP Ricinus communis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.15 0.2
-
 CTP pH 7.5 Ricinus communis
2.7.7.15 0.37
-
 choline phosphate pH 7.5 Ricinus communis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.15 40000
-
 4 * 40000, SDS-PAGE Ricinus communis
2.7.7.15 155000
-
 gel filtration Ricinus communis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.15 CTP + choline phosphate Ricinus communis catalyzes a major rate-limiting step in the biosynthesis of phosphocholine diphosphate + CDPcholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.15 Ricinus communis
-
 castor bean, L- var. Hale
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.15
-
 Ricinus communis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.15 endosperm
-
 Ricinus communis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.15 1.518
-
-
 Ricinus communis

Storage Stability

EC Number Storage Stability Organism
2.7.7.15 -20°C, enzyme concentrated to a small volume, 50% glycerol, 20% loss of activity after 2 weeks Ricinus communis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.15 CTP + choline phosphate
-
 Ricinus communis diphosphate + CDPcholine
-
 ?
2.7.7.15 CTP + choline phosphate catalyzes a major rate-limiting step in the biosynthesis of phosphocholine Ricinus communis diphosphate + CDPcholine
-
 ?
2.7.7.15 CTP + phosphomonomethylethanolamine
-
 Ricinus communis diphosphate + CDPmethylethanolamine
-
 ?
2.7.7.15 additional information no substrates: ethanolamine phosphate and phosphodimethylethanolamine Ricinus communis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.15 tetramer 4 * 40000, SDS-PAGE Ricinus communis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.15 7 7.5
-
 Ricinus communis
2.7.7.15 7.5
-
-
 Ricinus communis