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Literature summary extracted from

  • Wang, X.; Moore, T.S.
    Partial purification and characterization of CTP:cholinephosphate cytidylyltransferase from castor bean endosperm (1989), Arch. Biochem. Biophys., 274, 338-347.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.15 ATP extent of inhibition is dependent on preincubation time, temperature and Mg2+ and Ca2+ concentration Ricinus communis
2.7.7.15 phosphate 50% loss of activity at 10 mM Ricinus communis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.15 0.37
-
 CTP pH 7.6, 30°C Ricinus communis
2.7.7.15 1.1
-
 choline phosphate pH 7.6, 30°C Ricinus communis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.15 Ca2+
-
 Ricinus communis
2.7.7.15 Mg2+ required Ricinus communis
2.7.7.15 Mg2+ optimum concentration around 2 mM Ricinus communis

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.15 Ricinus communis
-
 castor bean, L- var. Hale
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.15
-
 Ricinus communis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.15 endosperm postgermination Ricinus communis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.15 0.6024
-
-
 Ricinus communis

Storage Stability

EC Number Storage Stability Organism
2.7.7.15 -20°C, stable for more than 1 month in presence of 20% glycerol Ricinus communis
2.7.7.15 4°C, 30% loss of activity after 1 week Ricinus communis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.15 CTP + choline phosphate
-
 Ricinus communis diphosphate + CDPcholine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.15 35
-
-
 Ricinus communis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.15 6.3
-
 assay at Ricinus communis
2.7.7.15 7 9
-
 Ricinus communis