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Literature summary extracted from

  • Choy, P.C.; Lim, P.H.; Vance, D.E.
    Purification and characterization of CTP: cholinephosphate cytidylytransferase from rat liver cytosol (1977), J. Biol. Chem., 252, 7673-7677.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.15 freezing and thawing once causes more than 90% loss of activity Rattus norvegicus
2.7.7.15 purified enzyme is unstable in presence of DTT. CTP-Mg2+ or bovine serum albumin do not significantly stabilize the enzyme Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.15 0.004
-
 diphosphate L-form, pH 6.4, 37°C Rattus norvegicus
2.7.7.15 0.007
-
 diphosphate H-form, pH 6.4, 37°C Rattus norvegicus
2.7.7.15 0.167
-
 choline phosphate L-form, pH 6.4, 37°C Rattus norvegicus
2.7.7.15 0.18
-
 choline phosphate H-form, pH 6.4, 37°C Rattus norvegicus
2.7.7.15 0.208
-
 CTP L-form, pH 6.4, 37°C Rattus norvegicus
2.7.7.15 0.21
-
 CDPcholine L-form, pH 6.4, 37°C Rattus norvegicus
2.7.7.15 0.296
-
 CTP H-form, pH 6.4, 37°C Rattus norvegicus
2.7.7.15 0.64
-
 CDPcholine H-form, pH 6.4, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.15 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.15 200000
-
 L-form of enzyme, gel filtration, aggregates in the cytosol to form high molecular weight species (H-form) with a median value of 1200000 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.15 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.15 affinity chromatography, 2 forms: L-form and H-form, L-form: major species in fresh cytosol, H-form: consists of multiple copies of L-form Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.15 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.15 0.106
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.15 CTP + choline phosphate highly specific for phosphocholine Rattus norvegicus diphosphate + CDPcholine
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.15 7
-
-
 Rattus norvegicus