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Literature summary extracted from

  • Sundler, R.
    Ethanolaminephosphate cytidylyltransferase. Purification and characterization of the enzyme from rat liver (1975), J. Biol. Chem., 250, 8585-8590.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.14 Reducing agent like DTT required Rattus norvegicus
2.7.7.14 Reducing agent cysteine or 2-mercaptoethanol are ineffective Rattus norvegicus

General Stability

EC Number General Stability Organism
2.7.7.14 bovine serum albumin, 2% w/v, 1 mM CTP and 20 mM Mg2+, or 10% v/v glycerol stabilizes Rattus norvegicus
2.7.7.14 enzyme in crude postmicrosomal supernatant is quite stable towards freezing and thawing, but the highly purified enzyme loses 85-90% of its activity when frozen and thawed twice Rattus norvegicus
2.7.7.14 omission of DTT from buffers used in the later steps of purification results in severe loss of activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.14 CDP-ethanolamine
-
 Rattus norvegicus
2.7.7.14 CTP at concentrations exceeding that of Mg2+ Rattus norvegicus
2.7.7.14 diphosphate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.14 0.053
-
 CTP pH 7.8, 37°C Rattus norvegicus
2.7.7.14 0.065
-
 Ethanolamine phosphate pH 7.8, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.14 cytosol postmicrosomal supernatant Rattus norvegicus 5829
-
2.7.7.14 additional information
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.14 Ca2+ activation, 10% of the activity with Mg2+ Rattus norvegicus
2.7.7.14 Co2+ activation, 10% of the activity with Mg2+ Rattus norvegicus
2.7.7.14 Mg2+ absolute requirement for a divalent cation Rattus norvegicus
2.7.7.14 Mg2+ 5-10 mM most efficient Rattus norvegicus
2.7.7.14 Mn2+ activation, optimal concentration: 2-4 mM, 50% of the activity with Mg2+ Rattus norvegicus
2.7.7.14 additional information no activation by Ba2+, Zn2+, Cd2+, Ni2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.14 100000 120000 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.14 CTP + ethanolamine phosphate Rattus norvegicus catalyzes a central step in phosphatidylethanolamine synthesis diphosphate + CDP-ethanolamine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.14 Rattus norvegicus
-
 male
-
2.7.7.14 Rattus norvegicus
-
 Sprague-Dawley rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.14
-
 Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.14 CTP + ethanolamine phosphate = diphosphate + CDP-ethanolamine ordered reaction mechanism Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.14 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.14 1.13
-
 purified enzyme Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.7.14 0-4°C, pH 7.5-9.0, in presence of DTT, stable for weeks Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.14 CTP + ethanolamine phosphate
-
 Rattus norvegicus diphosphate + CDP-ethanolamine
-
 r
2.7.7.14 CTP + ethanolamine phosphate catalyzes a central step in phosphatidylethanolamine synthesis Rattus norvegicus diphosphate + CDP-ethanolamine
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.14 ? x * 49000-50000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.14 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.14 6
-
 one sharp optimum at pH 7.8 and one with a lower maximal activity around pH 6.0 Rattus norvegicus
2.7.7.14 7.8
-
 one sharp optimum at pH 7.8 and one with a lower maximal activity around pH 6.0 Rattus norvegicus