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Literature summary extracted from

  • Wu, B.; Zhang, Y.; Zheng, R.; Guo, C.; Wang, P.G.
    Bifunctional phosphomannose isomerase/GDP-D-mannose pyrophosphorylase is the point of control for GDP-D-mannose biosynthesis in Helicobacter pylori (2002), FEBS Lett., 519, 87-92.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.13 expression in Escherichia coli Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.13 0.022
-
 alpha-D-mannose 1-phosphate pH 8.0, 37°C, cosubstrate GTP Helicobacter pylori
2.7.7.13 0.1
-
 GDPmannose pH 8.0, 37°C, cosubstrate diphosphate Helicobacter pylori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.13 Mg2+ required for activity Helicobacter pylori
2.7.7.13 Mn2+ 30% of activity with Mg2+ Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.13 55000
-
 x * 55000, SDS-PAGE Helicobacter pylori
2.7.7.13 55303
-
 x * 55303, deduced from nucleotide sequence Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.13 GTP + alpha-D-mannose 1-phosphate Helicobacter pylori enzyme regulates GDP-D-mannose synthesis through feedback inhibition GDPmannose + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.13 Helicobacter pylori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.13 recombinant enzyme, Ni-nitrilotriacetic acid column, Superdex 200 Helicobacter pylori

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.13 GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose bifunctional enzyme with both phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activity Helicobacter pylori
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Helicobacter pylori GDPmannose + diphosphate
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate enzyme regulates GDP-D-mannose synthesis through feedback inhibition Helicobacter pylori GDPmannose + diphosphate
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Helicobacter pylori diphosphate + GDP-mannose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.13 ? x * 55000, SDS-PAGE Helicobacter pylori
2.7.7.13 ? x * 55303, deduced from nucleotide sequence Helicobacter pylori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.13 10.3
-
 alpha-D-mannose 1-phosphate pH 8.0, 37°C, cosubstrate GTP Helicobacter pylori
2.7.7.13 22.1
-
 GDPmannose pH 8.0, 37°C, cosubstrate diphosphate Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.13 7
-
 GDP-mannose synthesis, forward reaction is very sensitive to pH fluctuation Helicobacter pylori
2.7.7.13 7 9 GTP synthesis Helicobacter pylori