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Literature summary extracted from

  • Ning, B.; Elbein, A.D.
    Cloning, expression and characterization of the pig liver GDP-mannose pyrophosphorylase: evidence that GDP-mannose and GDP-Glc pyrophosphorylases are different proteins (2000), Eur. J. Biochem., 267, 6866-6874.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.13 expression of GST-GDPmannose pyrophosphorylase fusion protein in Escherichia coli Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.13 0.000039
-
 GDPmannose pH 7.5, 37°C, recombinant beta subunit of GDPmannose pyrophosphorylase, cosubstrate diphosphate Sus scrofa
2.7.7.13 0.2
-
 mannose 1-phosphate pH 7.5, 37°C, recombinant beta subunit of GDPmannose pyrophosphorylase, cosubstrate GTP Sus scrofa
2.7.7.13 0.36
-
 GTP pH 7.5, 37°C, recombinant beta subunit of GDPmannose pyrophosphorylase, cosubstrate mannose 1-phosphate Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.13 Mg2+ recombinant beta subunit of GDPmannose pyrophosphorylase, 50% of activation with Mn2+ Sus scrofa
2.7.7.13 Mn2+ best metal activator of recombinant beta subunit of GDPmannose pyrophosphorylase, maximal activation at 0.5 mM Sus scrofa
2.7.7.13 additional information recombinant beta subunit of GDPmannose pyrophosphorylase is not activated by Co2+, Zn2+, Cu2+ and Fe2+ Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.13 43000
-
 43000 Da alpha subunit or a combinantion of alpha and beta subunit constitutes the GDPglucose pyrophosphorylase activity, the 37000 Da beta subunit constituts the GDPmannose pyrophosphorylase activity Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.13 GTP + alpha-D-mannose 1-phosphate Sus scrofa
-
 GDPmannose + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.13 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.13 ATP + alpha-D-mannose 1-phosphate recombinant beta subunit of GDPmannose pyrophosphorylase, 18% of activity with GTP Sus scrofa ADPmannose + diphosphate
-
 ?
2.7.7.13 CTP + alpha-D-mannose 1-phosphate recombinant beta subunit of GDPmannose pyrophosphorylase, 7% of activity with GTP Sus scrofa diphosphate + CDP-alpha-D-mannose
-
 ?
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Sus scrofa GDPmannose + diphosphate
-
 ?
2.7.7.13 GTP + alpha-D-mannose 1-phosphate recombinant 37000 Da beta subunit of GDP-mannose pyrophosphorylase has only little GDPglucose synthetic activity but high GDP mannose synthetic activity Sus scrofa GDPmannose + diphosphate
-
 ?
2.7.7.13 ITP + alpha-D-mannose 1-phosphate recombinant beta subunit of GDPmannose pyrophosphorylase, 20% of activity with GTP Sus scrofa IDPmannose + diphosphate
-
 ?
2.7.7.13 UTP + alpha-D-mannose 1-phosphate recombinant beta subunit of GDPmannose pyrophosphorylase, 12% of activity with GTP Sus scrofa UDPmannose + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.13 ?
-
 Sus scrofa
2.7.7.13 More 43000 Da alpha subunit or a combinantion of alpha and beta subunit constitutes the GDPglucose pyrophosphorylase activity, the 37000 Da beta subunit constituts the GDPmannose pyrophosphorylase activity Sus scrofa