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Literature summary extracted from

  • Fey, S.; Elling, L.; Kragl, U.
    The cofactor Mg2+ - a key switch for effective continuous enzymic production of GDP-mannose using recombinant GDP-mannose pyrophosphorylase (1998), Carbohydr. Res., 305, 475-481.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.13 expression in Escherichia coli strain BL21(DE3)pLysS Salmonella enterica

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.13 ammonium sulfate
-
 Salmonella enterica
2.7.7.13 diphosphate strong product inhibition Salmonella enterica
2.7.7.13 GDP-mannose strong product inhibition Salmonella enterica
2.7.7.13 Mg2+
-
 Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.13 0.002
-
 Mg2+ pH 8.0, 25°C Salmonella enterica
2.7.7.13 0.015
-
 alpha-D-mannose 1-phosphate pH 8.0, 25°C Salmonella enterica
2.7.7.13 0.04
-
 GTP pH 8.0, 25°C Salmonella enterica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.13 Mg2+ activity depends on the ratio of Mg2+ and GTP, maximum activity at a ratio of about 1 Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.13 GTP + alpha-D-mannose 1-phosphate Salmonella enterica
-
 GDPmannose + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.13 Salmonella enterica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.13 recombinant enzyme, ultrafiltration, Q-Sepharose Salmonella enterica

Storage Stability

EC Number Storage Stability Organism
2.7.7.13 4°C, ammonium sulfate suspension, several months, no loss of activity Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Salmonella enterica GDPmannose + diphosphate
-
 ?
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Salmonella enterica diphosphate + GDP-mannose
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.13 0.001
-
 Mg2+ pH 8.0, 25°C Salmonella enterica
2.7.7.13 0.009
-
 GDP-mannose pH 8.0, 25°C Salmonella enterica
2.7.7.13 0.016
-
 diphosphate pH 8.0, 25°C Salmonella enterica
2.7.7.13 0.85
-
 ammonium sulfate
-
 Salmonella enterica