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Literature summary extracted from

  • Gross, W.; Schnarrenberger, C.
    Purification and characterization of a galactose-1-phosphate: UDP-glucose uridyltransferase from the red alga Galdieria sulphuraria (1995), Eur. J. Biochem., 234, 258-263.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.12 stable to freezing and thawing, purified and crude Galdieria sulphuraria

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.12 additional information no inhibition by NADH, NAD+, sodium diphosphate Galdieria sulphuraria

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.12 0.02
-
 UDP-glucose pH 8.5, 22°C Galdieria sulphuraria
2.7.7.12 0.03
-
 UDP-galactose pH 7.5, 22°C Galdieria sulphuraria
2.7.7.12 0.105
-
 alpha-D-glucose 1-phosphate pH 7.5, 22°C Galdieria sulphuraria
2.7.7.12 0.4
-
 alpha-D-galactose 1-phosphate pH 8.5, 22°C Galdieria sulphuraria

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.12 42000
-
 2 * 42000, SDS-PAGE Galdieria sulphuraria
2.7.7.12 60000
-
 gel filtration Galdieria sulphuraria
2.7.7.12 82000
-
 sedimentation equilibrium centrifugation Galdieria sulphuraria

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.12 UDP-glucose + alpha-D-galactose 1-phosphate Galdieria sulphuraria involved in conversion of galactose into alpha-D-glucose 1-phosphate, Leloir pathway alpha-D-glucose 1-phosphate + UDP-galactose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.12 Galdieria sulphuraria
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.12 about 1800fold to homogeneity Galdieria sulphuraria

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.12 137
-
 purified enzyme Galdieria sulphuraria

Storage Stability

EC Number Storage Stability Organism
2.7.7.12 -20°C, stable Galdieria sulphuraria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.12 additional information no activity with UDP-xylose, ADP-glucose, and galactose 6-phosphate Galdieria sulphuraria ?
-
 ?
2.7.7.12 UDP-glucose + alpha-D-galactose 1-phosphate highly specific for the substrates Galdieria sulphuraria alpha-D-glucose 1-phosphate + UDP-galactose
-
 r
2.7.7.12 UDP-glucose + alpha-D-galactose 1-phosphate involved in conversion of galactose into alpha-D-glucose 1-phosphate, Leloir pathway Galdieria sulphuraria alpha-D-glucose 1-phosphate + UDP-galactose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.12 dimer 2 * 42000, SDS-PAGE Galdieria sulphuraria

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.12 22
-
 assay at Galdieria sulphuraria

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.12 additional information
-
 activation energy, temperature profile Galdieria sulphuraria

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.12 50
-
 half-life: 60 min Galdieria sulphuraria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.12 7 9 broad Galdieria sulphuraria