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Literature summary extracted from

  • Williams, V.P.; Helmer, G.R.; Fried, C.
    Human galactose-1-phosphate uridylyltransferase: purification and comparison of the red blood cell and placental enzymes (1982), Arch. Biochem. Biophys., 216, 503-511.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.12 UDP-glucose stabilizes during gel filtration Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.12 0.011
-
 alpha-D-galactose 1-phosphate with UDP-glucose Homo sapiens
2.7.7.12 0.015
-
 UDP-glucose erythrocytes Homo sapiens
2.7.7.12 0.021
-
 alpha-D-galactose 1-phosphate erythrocytes Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.12 45000
-
 x * 45000, SDS-PAGE Homo sapiens
2.7.7.12 67000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.12 UDP-glucose + alpha-D-galactose 1-phosphate Homo sapiens 2nd step of Leloir pathway alpha-D-glucose 1-phosphate + UDP-galactose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.12 Homo sapiens
-
 3 isozymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.12 partial from erythrocyte, several isozymes Homo sapiens
2.7.7.12 placental enzyme to homogeneity, coelutes with a 67 kDa glycoprotein Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.12 erythrocyte
-
 Homo sapiens
-
2.7.7.12 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.12 44
-
 erythrocytes Homo sapiens
2.7.7.12 92.2
-
 purified enzyme from placenta Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.12 UDP-glucose + alpha-D-galactose 1-phosphate
-
 Homo sapiens alpha-D-glucose 1-phosphate + UDP-galactose
-
 r
2.7.7.12 UDP-glucose + alpha-D-galactose 1-phosphate 2nd step of Leloir pathway Homo sapiens alpha-D-glucose 1-phosphate + UDP-galactose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.12 ? x * 45000, SDS-PAGE Homo sapiens
2.7.7.12 More a second band of MW 22 kDa is found in SDS-PAGE, which shows no enzyme activity Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.7.12 uridine diphosphoglucose:alpha-D-galactose-1-phosphate uridilyltransferase
-
 Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.12 Homo sapiens 3 placental isozymes 5.86 5.72