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Literature summary extracted from

  • Saito, S.; Ozutsumi, M.; Kurahashi, K.
    Galactose 1-phosphate uridylyltransferase of Escherichia coli. II. Further purification and characterization (1967), J. Biol. Chem., 242, 2362-2368.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.12 cysteine requirement Escherichia coli

General Stability

EC Number General Stability Organism
2.7.7.12 freeze-thawing inactivates purified enzyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.12 additional information
-
 additional information kinetics Escherichia coli
2.7.7.12 0.31
-
 UDP-galactose pH 8.8, 25°C Escherichia coli
2.7.7.12 0.41
-
 UDP-glucose pH 8.8, 25°C Escherichia coli
2.7.7.12 0.5
-
 alpha-D-glucose 1-phosphate pH 8.8, 25°C Escherichia coli
2.7.7.12 1.54
-
 alpha-D-galactose 1-phosphate pH 8.8, 25°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.12 41000
-
 2 * 41000, urea/mercaptoethanol treated and S-carboxymethylated enzyme, sedimentation equilibrium centrifugation in 5 M guanidine hydrochloride Escherichia coli
2.7.7.12 80000
-
 low speed sedimentation equilibrium Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.12 Escherichia coli
-
-
-
2.7.7.12 Escherichia coli
-
 operator-constitutive mutant strain C81-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.12 to homogeneity Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.12 209
-
 purified enzyme Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.7.12 0°C, 10% loss of activity per week Escherichia coli
2.7.7.12 0°C, 5 mM potassium phosphate buffer, pH 7, 20 mg/ml bovine serum albumin, 5 h stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.12 additional information no activity with fructose 1-phosphate and galactose 6-phosphate Escherichia coli ?
-
 ?
2.7.7.12 additional information no activity with ADP-glucose or GDP-glucose Escherichia coli ?
-
 ?
2.7.7.12 additional information no activity with ribose 1-phosphate and mannose 6-phosphate Escherichia coli ?
-
 ?
2.7.7.12 TDP-glucose + alpha-D-galactose 1-phosphate no activity Escherichia coli alpha-D-glucose 1-phosphate + TDP-galactose
-
 ?
2.7.7.12 UDP-glucose + alpha-D-galactose 1-phosphate highly specific for the substrates Escherichia coli alpha-D-glucose 1-phosphate + UDP-galactose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.12 dimer 2 * 41000, urea/mercaptoethanol treated and S-carboxymethylated enzyme, sedimentation equilibrium centrifugation in 5 M guanidine hydrochloride Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.12 25
-
 assay at Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.12 additional information
-
 activation energy, temperature profile Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.12 278
-
 alpha-D-galactose 1-phosphate pH 8.8, 25°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.12 8.5 9
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.12 7.2 9.8 about half-maximal activity at pH 7.2 and about 70% of maximal activity at pH 9.8 Escherichia coli