Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fehr, S.; Richter, D.
    Stringent response of Bacillus stearothermophilus: evidence for the existence of two distinct guanosine 3,5-polyphosphate synthetases (1981), J. Bacteriol., 145, 68-73.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.6.5 additional information (p)ppGpp synthetase II does not Geobacillus stearothermophilus
2.7.6.5 mRNA (p)ppGpp synthetase II does not Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.5 tetracycline not (p)ppGpp synthetase II Geobacillus stearothermophilus
2.7.6.5 Thiostrepton not (p)ppGpp synthetase II Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.5 0.05
-
 ATP synthetase II, pH 7.8, 37°C Geobacillus stearothermophilus
2.7.6.5 2
-
 ATP synthetase I, pH 7.8, 37°C Geobacillus stearothermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.6.5 cytosol (p)ppGpp synthetase II Geobacillus stearothermophilus 5829
-
2.7.6.5 ribosome (p)ppGpp synthetase I Geobacillus stearothermophilus 5840
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.5 74000
-
 synthetase II, sucrose density gradient centrifugation Geobacillus stearothermophilus
2.7.6.5 86000
-
 synthetase I, sucrose density gradient centrifugation Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.5 ATP + GTP Geobacillus stearothermophilus
-
 AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?
2.7.6.5 additional information Geobacillus stearothermophilus (p)ppGpp synthetase II is responsible for (p)ppGpp accumulation during carbon source downshift ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.5 Geobacillus stearothermophilus
-
 2 different enzymes: (p)ppGpp synthetase I, (p)ppGpp synthetase II
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.5 ATP + GTP
-
 Geobacillus stearothermophilus AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?
2.7.6.5 additional information (p)ppGpp synthetase II is responsible for (p)ppGpp accumulation during carbon source downshift Geobacillus stearothermophilus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.5 37
-
 assay at Geobacillus stearothermophilus