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Literature summary extracted from

  • Strominger, J.L.; Heppel, L.A.; Maxwell, E.S.
    Nucleoside monophosphate kinases. Transphosphorilation between adenosine triphosphate and nucleoside monophosphates (1959), Biochim. Biophys. Acta, 32, 412-421.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.4 EDTA variable degree of activation Bos taurus
2.7.4.4 glutathione variable degree of activation Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.4 Ca2+ half as effective as Mg2+ at 4 mM Bos taurus
2.7.4.4 Mg2+ activity saturation at 4 mM Bos taurus
2.7.4.4 Mn2+ as effective as Mg2+ Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.4 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.4 partial, using ammonium sulfate precipitation Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.4 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.4 additional information
-
 description of assay method Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.4.4 -15°C, 3 weeks, 75% of its activity lost Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.4 ATP + UMP
-
 Bos taurus ADP + UDP
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.4 80
-
 completely inactivated at pH 7 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.4 6.5 7.5 10% of the activity at pH 5.3 Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.4.4 1.3
-
 inactivation in few min at 2°C Bos taurus