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Literature summary extracted from

  • Tsuboi, K.K.
    AMP (dAMP) kinase from human erythrocytes (1978), Methods Enzymol., 51, 467-473.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.4.3 Triton X-100, EDTA, dithiothreitol and electrolyte protect enzyme in dilute solution and against denaturation by heat or extreme pH-values Homo sapiens
2.7.4.3 unstable in dilute solution Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.3 0.076 0.083 AMP 25°C, pH 8.0 Homo sapiens
2.7.4.3 0.09 0.092 ADP3- 25°C, pH 8.0 Homo sapiens
2.7.4.3 0.09 0.092 ADP3- ATP production Homo sapiens
2.7.4.3 0.11
-
 ADP 25°C, pH 8.0 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.3 cytosol
-
 Homo sapiens 5829
-
2.7.4.3 additional information stroma-associated Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.3 Mg2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.3 21300
-
 sedimentation equilibrium Homo sapiens
2.7.4.3 23000
-
 1 * 23000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.3 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.3 2 allelozymes: AK1*1 and AK1*2 Homo sapiens
2.7.4.3 and their multiple forms Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.3 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.3 3200
-
 25°C, pH 8.0 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.3 ADP + ADP
-
 Homo sapiens ATP + AMP
-
 r
2.7.4.3 ATP + AMP no substrates are ATP/dGMP Homo sapiens ADP + ADP
-
 r
2.7.4.3 ATP + AMP no substrates are ATP/IMP Homo sapiens ADP + ADP
-
 r
2.7.4.3 ATP + AMP no substrates are dGTP/AMP Homo sapiens ADP + ADP
-
 r
2.7.4.3 ATP + AMP no substrates are ATP/UMP Homo sapiens ADP + ADP
-
 r
2.7.4.3 ATP + AMP no substrates are ATP/GMP Homo sapiens ADP + ADP
-
 r
2.7.4.3 ATP + CMP reaction at 1% the rate of AMP Homo sapiens ADP + ?
-
 ?
2.7.4.3 ATP + dAMP reaction at 10% the rate of AMP Homo sapiens ADP + dADP
-
 ?
2.7.4.3 CTP + AMP reaction at about 3% the rate of ATP Homo sapiens ADP + CDP
-
 ?
2.7.4.3 dATP + AMP reaction at about 50% the rate of ATP Homo sapiens dADP + ADP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.3 monomer 1 * 23000, SDS-PAGE Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.4.3 Homo sapiens
-
-
 9