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Literature summary extracted from

  • Jaffor Ullah, A.H.; Cirillo, V.P.
    Mycoplasma phosphoenolpyruvate-dependent sugar phosphotransferase system: purification and characterization of enzyme I (1977), J. Bacteriol., 131, 988-996.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.3.9 N-ethylmaleimide irreversible inactivation Mycoplasma capricolum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.3.9 soluble
-
 Mycoplasma capricolum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.9 44500
-
 alpha2,beta,gamma, 2 * 44500 + 1 * 62000 + 1 * 64500, SDS-PAGE, N-terminal amino acid sequence Mycoplasma capricolum
2.7.3.9 62000
-
 alpha2,beta,gamma, 2 * 44500 + 1 * 62000 + 1 * 64500, SDS-PAGE, N-terminal amino acid sequence Mycoplasma capricolum
2.7.3.9 64500
-
 alpha2,beta,gamma, 2 * 44500 + 1 * 62000 + 1 * 64500, SDS-PAGE, N-terminal amino acid sequence Mycoplasma capricolum
2.7.3.9 220000
-
 gel filtration Mycoplasma capricolum

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.9 Mycoplasma capricolum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.9
-
 Mycoplasma capricolum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.3.9 additional information
-
-
 Mycoplasma capricolum

Storage Stability

EC Number Storage Stability Organism
2.7.3.9 -20°C, phosphate buffer, pH 7.0, protein concentration 1 mg/ml, stable for at least 14 months Mycoplasma capricolum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.9 phosphoenolpyruvate + histidine-containing protein
-
 Mycoplasma capricolum pyruvate + phosphorylated histidine-containing protein
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.3.9 tetramer alpha2,beta,gamma, 2 * 44500 + 1 * 62000 + 1 * 64500, SDS-PAGE, N-terminal amino acid sequence Mycoplasma capricolum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.3.9 37
-
 assay at Mycoplasma capricolum