BRENDA - Enzyme Database

Kinetic properties of arginine phosphokinase from honeybees, Apis mellifera L. (Hymenoptera, Apidae)

Cheung, A.C.; Arch. Biochem. Biophys. 154, 28-39 (1973)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.3.3
ATP
product inhibition, competitive with ADP, noncompetitive with L-Arg
Apis mellifera
2.7.3.3
D-Arg
product inhibition, competitive with arginine phosphate and noncompetitive withg ADP
Apis mellifera
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.3.3
Ca2+
activates
Apis mellifera
2.7.3.3
Co2+
activates
Apis mellifera
2.7.3.3
Cu2+
activates
Apis mellifera
2.7.3.3
Mg2+
highest activity if ratio Mg2+:ATP is 1:1, synthesis of arginine phosphate; highest activity if the ratio Mg2+:ADP is 4:1, synthesis of ATP
Apis mellifera
2.7.3.3
Mn2+
4 mM, activation; less effective than Mg2+ in activation
Apis mellifera
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
36000
-
gel filtration
Apis mellifera
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.3.3
Apis mellifera
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.3.3
-
Apis mellifera
Reaction
EC Number
Reaction
Commentary
Organism
2.7.3.3
ATP + L-arginine = ADP + Nomega-phospho-L-arginine
rapid equilibrium random mechanism
Apis mellifera
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ATP + L-Arg
-
642453
Apis mellifera
ADP + Nomega-phospho-L-Arg
-
642453
Apis mellifera
r
2.7.3.3
ATP + L-arginine methyl ester
-
642453
Apis mellifera
ADP + Nomega-phospho-L-arginine methyl ester
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.3.3
45
-
synthesis of ATP and synthesis of N-phospho-L-Arg
Apis mellifera
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.3.3
7.1
7.2
synthesis of ATP
Apis mellifera
2.7.3.3
8.3
-
synthesis of N-phospho-L-arginine
Apis mellifera
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.3.3
5
8.2
pH 5.0: about 30% of maximal activity, pH 8.2: about 30% of maximal activity, ATP synthesis
Apis mellifera
2.7.3.3
6.3
9
pH 6.3: about 65% of maximal activity, pH 9.0: about 70% of maximal activity, synthesis of N-phospho-L-Arg
Apis mellifera
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.3.3
ATP
product inhibition, competitive with ADP, noncompetitive with L-Arg
Apis mellifera
2.7.3.3
D-Arg
product inhibition, competitive with arginine phosphate and noncompetitive withg ADP
Apis mellifera
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.3.3
Ca2+
activates
Apis mellifera
2.7.3.3
Co2+
activates
Apis mellifera
2.7.3.3
Cu2+
activates
Apis mellifera
2.7.3.3
Mg2+
highest activity if ratio Mg2+:ATP is 1:1, synthesis of arginine phosphate; highest activity if the ratio Mg2+:ADP is 4:1, synthesis of ATP
Apis mellifera
2.7.3.3
Mn2+
4 mM, activation; less effective than Mg2+ in activation
Apis mellifera
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
36000
-
gel filtration
Apis mellifera
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.3.3
-
Apis mellifera
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ATP + L-Arg
-
642453
Apis mellifera
ADP + Nomega-phospho-L-Arg
-
642453
Apis mellifera
r
2.7.3.3
ATP + L-arginine methyl ester
-
642453
Apis mellifera
ADP + Nomega-phospho-L-arginine methyl ester
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.3.3
45
-
synthesis of ATP and synthesis of N-phospho-L-Arg
Apis mellifera
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.3.3
7.1
7.2
synthesis of ATP
Apis mellifera
2.7.3.3
8.3
-
synthesis of N-phospho-L-arginine
Apis mellifera
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.3.3
5
8.2
pH 5.0: about 30% of maximal activity, pH 8.2: about 30% of maximal activity, ATP synthesis
Apis mellifera
2.7.3.3
6.3
9
pH 6.3: about 65% of maximal activity, pH 9.0: about 70% of maximal activity, synthesis of N-phospho-L-Arg
Apis mellifera