Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Haas, D.; Leisinger, T.
    N-acetylglutamate 5-phosphotransferase of Pseudomonas aeruginosa. Purification and ligand-directed association-dissociation (1975), Eur. J. Biochem., 52, 365-375.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.2.8 loss of activity on repeated freezing and thawing Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.8 Mg2+ required Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.8 29000
-
 x * 29000, SDS-PAGE Pseudomonas aeruginosa
2.7.2.8 230000
-
 gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.8 ATP + N-acetyl-L-glutamate Pseudomonas aeruginosa enzyme synthesis not repressed by exogenous L-arginine or its precursors ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?
2.7.2.8 ATP + N-acetyl-L-glutamate Pseudomonas aeruginosa second enzyme of arginine biosynthesis ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.8 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.8
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.8 additional information
-
-
 Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
2.7.2.8 -20°C, stable over extended periods Pseudomonas aeruginosa
2.7.2.8 4°C, 0.1 M phosphate buffer, stable Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.8 ATP + N-acetyl-L-glutamate
-
 Pseudomonas aeruginosa ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?
2.7.2.8 ATP + N-acetyl-L-glutamate enzyme synthesis not repressed by exogenous L-arginine or its precursors Pseudomonas aeruginosa ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?
2.7.2.8 ATP + N-acetyl-L-glutamate second enzyme of arginine biosynthesis Pseudomonas aeruginosa ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.8 ? x * 29000, SDS-PAGE Pseudomonas aeruginosa
2.7.2.8 More due to the capacity for self-association the enzyme can exist in different states of aggregation depending on the nature of the ligands and the concentration of phosphate buffer Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.8 25
-
 assay at Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.8 7
-
 assay at Pseudomonas aeruginosa