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Literature summary extracted from

  • Relton, J.M.; Bonner, P.L.R.; Wallsgrove, R.M.; Lea, P.J.
    Physical and kinetic properties of lysine-sensitive aspartate kinase purified from carrot cell suspension culture (1988), Biochim. Biophys. Acta, 953, 48-60.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.2.4 beta-Alanine 10% activation at 0.33 M Daucus carota

General Stability

EC Number General Stability Organism
2.7.2.4 stable up to. In the DEAE-Sephacel stage of purification, L-lysine and L-proline protect from heat inactivation Daucus carota

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.4 HgSO4 15% inhibition Daucus carota
2.7.2.4 iodosalicylate 20% inhibition Daucus carota
2.7.2.4 L-lysine
-
 Daucus carota
2.7.2.4 N-ethylmaleimide 17% inhibition Daucus carota
2.7.2.4 p-chloromercuribenzoate
-
 Daucus carota
2.7.2.4 Rose bengal
-
 Daucus carota
2.7.2.4 S-adenosyl-L-methionine potentiates inhibition by lysine Daucus carota

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.4 0.6
-
 ATP pH 7.5, 30°C Daucus carota
2.7.2.4 2.35
-
 L-aspartate pH 7.5, 30°C Daucus carota

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.4 additional information does not require monovalent cations for optimum activity Daucus carota

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.4 100000
-
 sucrose density gradient centrifugation, peak 1 Daucus carota
2.7.2.4 150000
-
 sucrose density gradient centrifugation, peak 2 Daucus carota
2.7.2.4 230000
-
 sucrose density gradient centrifugation, peak 3 Daucus carota
2.7.2.4 246000
-
 gel filtration, Superose 6 Daucus carota
2.7.2.4 253000
-
 gel filtration, non-denaturing electrophoresis, 4-20% polyacrylamide gradient gels Daucus carota
2.7.2.4 258000
-
 gel filtration, Superose 12 Daucus carota

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.4 Daucus carota
-
 carrot
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.4
-
 Daucus carota

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.4 cell suspension culture
-
 Daucus carota
-

Storage Stability

EC Number Storage Stability Organism
2.7.2.4 -20°C, can be stored without any noticeable loss in activity Daucus carota
2.7.2.4 use of bicine buffer and addition of sorbitol, bovine serum albumin or poly(ethyleneglycol) 6000 allows storage of the partially purified enzyme as a freeze-dried powder with relatively small loss of activity upon rehydration Daucus carota

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.4 ATP + L-aspartate
-
 Daucus carota ADP + 4-phospho-L-aspartate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.4 7.5
-
-
 Daucus carota

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.2.4 Daucus carota in presence of 0.1 mM L-lysine, determined by chromatofocusing
-
 4.43
2.7.2.4 Daucus carota in absence of L-lysine, determined by chromatofocusing
-
 5.16