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Literature summary extracted from

  • Mukherjee, K.; Ghosh, S.; Ray, M.; Ray, S.
    Purification and characterization of 3-phosphoglycerate kinase from Ehrlich ascites carcinoma cells (2002), Indian J. Biochem. Biophys., 39, 332-341.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.2.3 additional information no effect by glucose 6-phosphate, fructose 6-phosphate, fructose 1,6-phosphate, pyruvate, phosphoenolpyruvate and lactate Mus musculus

General Stability

EC Number General Stability Organism
2.7.2.3 optimal stabilization at 1 mM EDTA and 5 mM 2-mercaptoethanol Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.3 ADP inhibitory in forward reaction Mus musculus
2.7.2.3 citrate 40% inhibition at 50 mM Mus musculus
2.7.2.3 additional information no effect by glucose 6-phosphate, fructose 6-phosphate, fructose 1,6-phosphate, pyruvate, phosphoenolpyruvate and lactate Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.3 additional information
-
 additional information Km values at different pH-values Mus musculus
2.7.2.3 0.11
-
 ATP pH 6.9, 25°C Mus musculus
2.7.2.3 0.2
-
 3-phospho-D-glycerate pH 6.9, 25°C Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.3 divalent cation Mg2+ or Mn2+ Mus musculus
2.7.2.3 Mg2+ 0.8 m Mg2+ and 0.5 mM Mn2+ are optimal Mus musculus
2.7.2.3 Mn2+ 0.8 mM Mg2+ and 0.5 mM Mn2+ are optimal Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 47000
-
 gel filtration Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Mus musculus
-
 Swiss albino mice
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3 876fold, to homogeneity Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.3 Ehrlich ascites carcinoma cell from intraperitoneal cavity Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.3 169
-
 purified enzyme Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Mus musculus ADP + 1,3-diphosphoglycerate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.2.3 monomer 1 * 47000, SDS-PAGE Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.3 25
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.3 6 7.5
-
 Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.2.3 5.4 8 30% activity at pH 5.4, 60% activity at pH 8.0 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Mus musculus
2.7.2.3 ATP required as phosphate donor Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.2.3 0.08
-
 ADP pH 6.5, 25°C, versus ATP Mus musculus
2.7.2.3 0.175
-
 ADP pH 6.5, 25°C, versus 3-phospho-D-glycerate Mus musculus