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Literature summary extracted from

  • Mizuno, Y.; Ohshima, M.; Yao, Y.; Shibasaki, R.; Takahashi, R.; Tokuyama, T.
    Purification and comparison of phosphoglycerate kinases from nitrifying bacteria (1998), J. Ferment. Bioeng., 86, 346-350.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.3 p-chloromercuribenzoate strong Nitrosomonas europaea
2.7.2.3 p-chloromercuribenzoate strong Nitrosomonas sp. K1
2.7.2.3 SDS strong Nitrosomonas europaea
2.7.2.3 SDS strong Nitrosomonas sp. K1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 43400
-
 1 * 43400 Rhodopseudomonas palustris
2.7.2.3 43700
-
 1 * 43700 Nitrosomonas sp. K1
2.7.2.3 44300
-
 1 * 44300 Nitrosomonas sp. TNO632
2.7.2.3 44600
-
 1 * 44600 Nitrosomonas europaea
2.7.2.3 46100
-
 1 * 46100 Nitrobacter winogradskyi

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Nitrobacter winogradskyi
-
 IFO 14297
-
2.7.2.3 Nitrosomonas europaea
-
 ATCC 25978T
-
2.7.2.3 Nitrosomonas sp. K1
-
-
-
2.7.2.3 Nitrosomonas sp. TNO632
-
-
-
2.7.2.3 Rhodopseudomonas palustris
-
 strain JCM2524
-
2.7.2.3 Rhodopseudomonas palustris JCM 2524
-
 strain JCM2524
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3 to homogeneity Rhodopseudomonas palustris
2.7.2.3 to homogeneity Nitrosomonas europaea
2.7.2.3 to homogeneity Nitrobacter winogradskyi
2.7.2.3 to homogeneity Nitrosomonas sp. TNO632
2.7.2.3 to homogeneity Nitrosomonas sp. K1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Rhodopseudomonas palustris ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Nitrobacter winogradskyi ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Nitrosomonas sp. TNO632 ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate specific for ATP Nitrosomonas europaea ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate specific for ATP Nitrosomonas sp. K1 ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Rhodopseudomonas palustris JCM 2524 ADP + 1,3-diphosphoglycerate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.2.3 monomer 1 * 44600 Nitrosomonas europaea
2.7.2.3 monomer 1 * 44300 Nitrosomonas sp. TNO632
2.7.2.3 monomer 1 * 46100 Nitrobacter winogradskyi
2.7.2.3 monomer 1 * 43700 Nitrosomonas sp. K1
2.7.2.3 monomer 1 * 43400 Rhodopseudomonas palustris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.3 40
-
-
 Nitrosomonas sp. K1
2.7.2.3 50
-
-
 Nitrosomonas europaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.3 8 8.5
-
 Nitrosomonas europaea
2.7.2.3 8 8.5
-
 Nitrosomonas sp. K1

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.2.3 7 9 stable at 4°C for 48 h Rhodopseudomonas palustris
2.7.2.3 7 9 stable at 4°C for 48 h Nitrosomonas europaea
2.7.2.3 7 9 stable at 4°C for 48 h Nitrobacter winogradskyi
2.7.2.3 7 9 stable at 4°C for 48 h Nitrosomonas sp. TNO632
2.7.2.3 7 9 stable at 4°C for 48 h Nitrosomonas sp. K1

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Rhodopseudomonas palustris
2.7.2.3 ADP
-
 Nitrosomonas europaea
2.7.2.3 ADP
-
 Nitrobacter winogradskyi
2.7.2.3 ADP
-
 Nitrosomonas sp. TNO632
2.7.2.3 ADP
-
 Nitrosomonas sp. K1
2.7.2.3 ATP required as phosphate donor Rhodopseudomonas palustris
2.7.2.3 ATP required as phosphate donor Nitrosomonas europaea
2.7.2.3 ATP required as phosphate donor Nitrobacter winogradskyi
2.7.2.3 ATP required as phosphate donor Nitrosomonas sp. TNO632
2.7.2.3 ATP required as phosphate donor Nitrosomonas sp. K1