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Literature summary extracted from

  • Zomer, A.W.; Allert, S.; Chevalier, N.; Callens, M.; Opperdoes, F.R.; Michels, P.A.
    Purification and characterisation of the phosphoglycerate kinase isoenzymes of Trypanosoma brucei expressed in Escherichia coli (1998), Biochim. Biophys. Acta, 1386, 179-188.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.3 expression of the glycosomal enzyme lacking the C-terminal glycosome-specific 20-residue signal sequence, in Escherichia coli Trypanosoma brucei
2.7.2.3 overexpression of glycosomal and cytosolic isozymes in Escherichia coli BL21 Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.3 Ib1 isozymes are differntly sensitive Trypanosoma brucei
2.7.2.3 suramin isozymes are differently sensitive Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.3 0.21
-
 ATP recombinant cytosolic isozyme, pH 7.6, 25°C Trypanosoma brucei
2.7.2.3 0.28
-
 ATP native glycosomal isozyme, pH 7.6, 25°C Trypanosoma brucei
2.7.2.3 0.29
-
 ATP recombinant glycosomal isozyme, pH 7.6, 25°C Trypanosoma brucei
2.7.2.3 0.33
-
 ATP recombinant truncated glycosomal isozyme, pH 7.6, 25°C Trypanosoma brucei
2.7.2.3 0.46
-
 ATP native glycosomal isozyme, pH 7.6, 25°C Trypanosoma brucei
2.7.2.3 1.25
-
 3-phospho-D-glycerate native cytosolic isozyme, pH 7.6, 25°C Trypanosoma brucei
2.7.2.3 1.55
-
 3-phospho-D-glycerate native glycosomal isozyme, pH 7.6, 25°C Trypanosoma brucei
2.7.2.3 2
-
 3-phospho-D-glycerate recombinant glycosomal isozyme and native cytosoli isozyme, pH 7.6, 25°C Trypanosoma brucei
2.7.2.3 11.9
-
 3-phospho-D-glycerate recombinant truncated glycosomal isozyme, pH 7.6, 25°C Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.2.3 cytosol cytosolic isozyme Trypanosoma brucei 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 45000
-
 x * 45000, cytosolic and truncated glycosomal isozymes, SDS-PAGE Trypanosoma brucei
2.7.2.3 47000
-
 x * 47000, glycosomal isozyme, SDS-PAGE Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Trypanosoma brucei
-
 glycosomal PGKA and cytosolic PGKB isozymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3 2 isozymes and the truncated glycosomal isozyme, recombinant from Escherichia coli, to near homogeneity Trypanosoma brucei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.3 159
-
 purified recombinant truncated glycosomal isozyme Trypanosoma brucei
2.7.2.3 159
-
 purified recombinant cytosolic isozyme Trypanosoma brucei
2.7.2.3 1311
-
 purified recombinant glycosomal isozyme Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Trypanosoma brucei ADP + 1,3-diphosphoglycerate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.2.3 ? x * 47000, glycosomal isozyme, SDS-PAGE Trypanosoma brucei
2.7.2.3 dimer x * 45000, cytosolic and truncated glycosomal isozymes, SDS-PAGE Trypanosoma brucei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.3 25
-
-
 Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Trypanosoma brucei
2.7.2.3 ATP required as phosphate donor Trypanosoma brucei

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.2.3 Trypanosoma brucei native cytosolic isozyme, isoelectric focusing 6.8 6.3
2.7.2.3 Trypanosoma brucei recombinant cytosolic isozyme, isoelectric focussing
-
 6.5
2.7.2.3 Trypanosoma brucei truncated glycosomal isozyme, isoelectric focusing
-
 9.2
2.7.2.3 Trypanosoma brucei glycosomal isozyme, isoelectric focusing
-
 9.3