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Literature summary extracted from

  • Okonkwo, P.O.; Askari, A.; Korngold, L.
    Human erythrocyte phosphoglycerate kinase: purification, properties, and interaction with its antibody (1973), Biochim. Biophys. Acta, 321, 503-511.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.3 crystallization via precipitation with ammonium sulfate stepwise from 60% to 75% w/v at room temperature for 2 h, then 4°C, several days Homo sapiens

General Stability

EC Number General Stability Organism
2.7.2.3 crystalline enzyme is rather unstable, dried crystals or dilute aqueous solutions lose all activity within a few weeks Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.3 Ca2+ partial inhibition in presence of Mg2+ Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.3 Ca2+ can partially replace Mg2+ in activation Homo sapiens
2.7.2.3 Ca2+ 30% of the activity with Mg2+ Homo sapiens
2.7.2.3 divalent cation requires Mg2+ or Ca2+ Homo sapiens
2.7.2.3 Mg2+ required Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.3 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.3 580
-
 purified crystalline enzyme Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.7.2.3 4°C, crystals as a suspension in 3 M (NH4)2SO4, 1 mM sodium diphosphate, 1 mM EDTA Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate absolute specificity for 3-phospho-D-glycerate Homo sapiens ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 GTP + 3-phospho-D-glycerate 60% of the activity with ATP Homo sapiens GDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 ITP + 3-phospho-D-glycerate 64% of the activity with ATP Homo sapiens IDP + 1,3-diphosphoglycerate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.3 25
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Homo sapiens
2.7.2.3 ATP required as phosphate donor Homo sapiens
2.7.2.3 GTP
-
 Homo sapiens
2.7.2.3 ITP
-
 Homo sapiens