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Literature summary extracted from

  • Yoshida, A.
    Human phosphoglycerate kinase (1975), Methods Enzymol., 42C, 144-148.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.3 crystallization via precipitation with ammonium sulfate stepwise from 30-35% to 65% w/v within 24 h Homo sapiens

General Stability

EC Number General Stability Organism
2.7.2.3 freeze-drying inactivates Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.3 Mg2+ inhibition above 50 mM Homo sapiens
2.7.2.3 Mn2+ inhibition above 50 mM Homo sapiens
2.7.2.3 phosphate phosphate buffer Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.3 additional information
-
 additional information Km for ATP at different concentrations Homo sapiens
2.7.2.3 0.59
-
 3-phospho-D-glycerate pH 7.5, 25°C Homo sapiens
2.7.2.3 1.1
-
 3-phospho-D-glycerate at 0.1 M Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.3 divalent cation Mg2+ or Mn2+ Homo sapiens
2.7.2.3 Mg2+ required Homo sapiens
2.7.2.3 Mg2+ 5 mM Mg2+ or 10 mM Mn2+ required Homo sapiens
2.7.2.3 Mn2+ Mn2+ or Mg2+ required Homo sapiens
2.7.2.3 Mn2+ 5 mM Mg2+ or 10 mM Mn2+ required Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 49600
-
 sedimentation equilibrium method Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.3 blood
-
 Homo sapiens
-
2.7.2.3 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.3 650 700 purified enzyme, at 25°C Homo sapiens
2.7.2.3 1000
-
 purified enzyme, at 35°C Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.7.2.3 4°C, with 10 mg/ml bovine serum albumin, stable for at least 1 week Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Homo sapiens ADP + 1,3-diphosphoglycerate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.3 25
-
 assay at Homo sapiens
2.7.2.3 35
-
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.3 7.2 9
-
 Homo sapiens
2.7.2.3 7.5
-
 assay at Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.2.3 6.5 8 enzyme in crude hemolysate is stable at 4°C for at least 1 week, inactive below pH 6.0 Homo sapiens
2.7.2.3 6.5 8 crystallized enzyme, solved at a low concentration loses 10% activity per 24 h Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Homo sapiens
2.7.2.3 ATP required as phosphate donor Homo sapiens