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Literature summary extracted from

  • Lee, C.Y.
    3-Phosphoglycerate kinase isozymes and genetic variants from mouse (1982), Methods Enzymol., 90, 121-126.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.3 additional information
-
 additional information kinetics for 3-phospho-D-glycerate are biphasic Mus musculus
2.7.2.3 0.028
-
 3-phospho-D-glycerate isozyme PGK2C Mus musculus
2.7.2.3 0.038
-
 ATP isoenzyme PGK2C Mus musculus
2.7.2.3 0.039
-
 ADP isozyme PGK2C Mus musculus
2.7.2.3 0.15
-
 3-phospho-D-glycerate isozyme PGK2B, ADP, isoenzyme PGK2A Mus musculus
2.7.2.3 0.19
-
 ADP isozyme PGK2B Mus musculus
2.7.2.3 0.2
-
 3-phospho-D-glycerate isoenzyme PGK2A Mus musculus
2.7.2.3 0.22
-
 ATP isoenzyme PGK1 Mus musculus
2.7.2.3 0.23
-
 ADP isozyme PGK1 Mus musculus
2.7.2.3 0.37
-
 ATP isozyme PGK2B Mus musculus
2.7.2.3 0.37
-
 ATP ATP in form of MgATP2- Mus musculus
2.7.2.3 0.42
-
 ATP isozyme PGK2A Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.3 Mg2+
-
 Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 47000
-
 gel filtration Mus musculus
2.7.2.3 47000
-
 isoenzymes PGK1, PGK2A, PGK2B, PGK2C Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Mus musculus
-
 2 isozymes: X-chromosome encoded PGK1 and chromosome 17 encoded PGK2, 3 genetic variants of PGK2: PGK2A, PGK2B, PGK2C
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3 isoenzyme PGK1 and all 3 genetic variants of PGK2 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.3 additional information isozyme PGK1 is found in all somatic tissues Mus musculus
-
2.7.2.3 muscle
-
 Mus musculus
-
2.7.2.3 spermatozoon isozyme PGK2 Mus musculus
-
2.7.2.3 testis mature Mus musculus
-
2.7.2.3 testis isozyme PGK2 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.3 8.5
-
 purified isozyme PGK2C Mus musculus
2.7.2.3 430
-
 purified isozyme PGK2B Mus musculus
2.7.2.3 450
-
 purified isozyme PGK2A Mus musculus
2.7.2.3 500
-
 purified isozyme PGK1 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Mus musculus ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Mus musculus ADP + 3-phospho-D-glyceroyl phosphate
-
 r
2.7.2.3 CTP + 3-phospho-D-glycerate low activity Mus musculus CDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 dATP + 3-phospho-D-glycerate isozymes PGK2A, PGK2B, and PGK1 show low activity, isozyme PGK2C shows 119% of the activity with ATP Mus musculus dADP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 dGTP + 3-phospho-D-glycerate isozyme PGK2C with lower activity, all other isozymes show slightly lower activity compared to ATP Mus musculus dGDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 GTP + 3-phospho-D-glycerate
-
 Mus musculus GDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 UTP + 3-phospho-D-glycerate
-
 Mus musculus UDP + 1,3-diphosphoglycerate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.3 25
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.3 8
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Mus musculus
2.7.2.3 ATP required as phosphate donor Mus musculus
2.7.2.3 CTP
-
 Mus musculus
2.7.2.3 dATP
-
 Mus musculus
2.7.2.3 dGTP
-
 Mus musculus
2.7.2.3 GTP
-
 Mus musculus
2.7.2.3 UTP
-
 Mus musculus