EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.2.1 | cloned and hyper-expressed in Escherichia coli | Methanosarcina thermophila |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.2.1 | D148A | site-directed mutagenesis | Methanosarcina thermophila |
2.7.2.1 | D148E | site-directed mutagenesis | Methanosarcina thermophila |
2.7.2.1 | D148N | site-directed mutagenesis | Methanosarcina thermophila |
2.7.2.1 | E384A | site-directed mutagenesis | Methanosarcina thermophila |
2.7.2.1 | K14A | site-directed mutagenesis | Methanosarcina thermophila |
2.7.2.1 | K14R | site-directed mutagenesis | Methanosarcina thermophila |
2.7.2.1 | N211A | site-directed mutagenesis | Methanosarcina thermophila |
2.7.2.1 | N7A | site-directed mutagenesis | Methanosarcina thermophila |
2.7.2.1 | S10A | site-directed mutagenesis | Methanosarcina thermophila |
2.7.2.1 | S11A | site-directed mutagenesis | Methanosarcina thermophila |
2.7.2.1 | S12A | site-directed mutagenesis | Methanosarcina thermophila |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.2.1 | 1.5 | - |
ATP | - |
Methanosarcina thermophila | |
2.7.2.1 | 2.13 | - |
ATP | pH 7.4, 37°C, wild-type | Methanosarcina thermophila | |
2.7.2.1 | 3 | - |
ATP | pH 7.4, 37°C, mutant N211A | Methanosarcina thermophila | |
2.7.2.1 | 3.1 | - |
ATP | pH 7.4, 37°C, mutant D148A | Methanosarcina thermophila | |
2.7.2.1 | 3.2 | - |
ATP | pH 7.4, 37°C, mutant S10A | Methanosarcina thermophila | |
2.7.2.1 | 3.62 | - |
ATP | pH 7.4, 37°C, mutant K14R | Methanosarcina thermophila | |
2.7.2.1 | 3.7 | - |
ATP | pH 7.4, 37°C, mutant S12T | Methanosarcina thermophila | |
2.7.2.1 | 4.3 | - |
ATP | pH 7.4, 37°C, mutant S10T | Methanosarcina thermophila | |
2.7.2.1 | 4.5 | - |
ATP | pH 7.4, 37°C, mutant S11A | Methanosarcina thermophila | |
2.7.2.1 | 5 | - |
ATP | pH 7.4, 37°C, mutant N7D, D148E | Methanosarcina thermophila | |
2.7.2.1 | 5.2 | - |
ATP | pH 7.4, 37°C, mutant K14A | Methanosarcina thermophila | |
2.7.2.1 | 5.8 | - |
ATP | pH 7.4, 37°C, mutant S10T | Methanosarcina thermophila | |
2.7.2.1 | 6.8 | - |
ATP | pH 7.4, 37°C, mutant S11T | Methanosarcina thermophila | |
2.7.2.1 | 7 | - |
ATP | pH 7.4, 37°C, mutant N7A | Methanosarcina thermophila | |
2.7.2.1 | 7.3 | - |
ATP | pH 7.4, 37°C, mutant S12A | Methanosarcina thermophila | |
2.7.2.1 | 7.7 | - |
ATP | pH 7.4, 37°C, mutant E384A | Methanosarcina thermophila | |
2.7.2.1 | 48 | - |
acetate | pH 7.4, 37°C, wild-type | Methanosarcina thermophila | |
2.7.2.1 | 66 | - |
acetate | - |
Methanosarcina thermophila | |
2.7.2.1 | 111 | - |
acetate | pH 7.4, 37°C, mutant N211A | Methanosarcina thermophila | |
2.7.2.1 | 170 | - |
acetate | pH 7.4, 37°C, mutant D148A | Methanosarcina thermophila | |
2.7.2.1 | 200 | - |
acetate | pH 7.4, 37°C, mutant S11A | Methanosarcina thermophila | |
2.7.2.1 | 239 | - |
acetate | pH 7.4, 37°C, mutant K14R | Methanosarcina thermophila | |
2.7.2.1 | 266 | - |
acetate | pH 7.4, 37°C, mutant S10T | Methanosarcina thermophila | |
2.7.2.1 | 333 | - |
acetate | pH 7.4, 37°C, mutant S12T | Methanosarcina thermophila | |
2.7.2.1 | 390 | - |
acetate | pH 7.4, 37°C, mutant D148N | Methanosarcina thermophila | |
2.7.2.1 | 693 | - |
acetate | pH 7.4, 37°C, mutant N7D | Methanosarcina thermophila | |
2.7.2.1 | 814 | - |
acetate | pH 7.4, 37°C, mutant S10A | Methanosarcina thermophila | |
2.7.2.1 | 1573 | - |
acetate | pH 7.4, 37°C, mutant S11T | Methanosarcina thermophila |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.2.1 | Ca2+ | - |
Methanosarcina thermophila | |
2.7.2.1 | Mg2+ | - |
Methanosarcina thermophila | |
2.7.2.1 | Mn2+ | - |
Methanosarcina thermophila |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.1 | ATP + acetate | Methanosarcina thermophila | - |
ADP + acetyl phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.2.1 | Methanosarcina thermophila | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 0.28 | - |
mutant D148A | Methanosarcina thermophila |
2.7.2.1 | 3.4 | - |
mutant E384A | Methanosarcina thermophila |
2.7.2.1 | 9 | - |
mutant S10A | Methanosarcina thermophila |
2.7.2.1 | 13 | - |
mutant N7A | Methanosarcina thermophila |
2.7.2.1 | 19 | - |
mutant S12A | Methanosarcina thermophila |
2.7.2.1 | 102 | - |
mutant K14A | Methanosarcina thermophila |
2.7.2.1 | 324 | - |
mutant S11A | Methanosarcina thermophila |
2.7.2.1 | 539 | - |
mutant N211A | Methanosarcina thermophila |
2.7.2.1 | 584 | - |
wild-type | Methanosarcina thermophila |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.1 | ATP + acetate | - |
Methanosarcina thermophila | ADP + acetyl phosphate | - |
r |