EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.2.1 | wild-type and mutant acetate kinase genes subcloned in to the T7-based expression vector pET15b | Methanosarcina thermophila |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.2.1 | E384A | site-directed mutagenesis | Methanosarcina thermophila |
2.7.2.1 | E384D | site-directed mutagenesis | Methanosarcina thermophila |
2.7.2.1 | E384Q | site-directed mutagenesis | Methanosarcina thermophila |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.2.1 | additional information | not inactivated by N-ethylmaleimide | Methanosarcina thermophila | |
2.7.2.1 | N-ethylmaleimide | - |
Methanosarcina thermophila |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.2.1 | 0.7 | - |
ATP | pH 7.0, 20°C, mutant E334A, produced in E. coli | Methanosarcina thermophila | |
2.7.2.1 | 1 | - |
ATP | pH 7.0, 20°C, unaltered enzyme, produced in E. coli | Methanosarcina thermophila | |
2.7.2.1 | 1.1 | - |
ATP | pH 7.0, 20°C, mutant E32A, produced in E. coli | Methanosarcina thermophila | |
2.7.2.1 | 1.4 | - |
ATP | pH 7.0, 20°C, mutants E97A, E97D, E97Q, produced in E. coli | Methanosarcina thermophila | |
2.7.2.1 | 1.6 | - |
ATP | pH 7.0, 20°C, mutant E385C, produced in E. coli | Methanosarcina thermophila | |
2.7.2.1 | 2 | - |
ATP | pH 7.0, 20°C, mutant E385Q, produced in E. coli | Methanosarcina thermophila | |
2.7.2.1 | 2.3 | - |
acetate | pH 7.0, 20°C, mutant E97A, produced in E. coli | Methanosarcina thermophila | |
2.7.2.1 | 2.8 | - |
ATP | - |
Methanosarcina thermophila | |
2.7.2.1 | 4 | - |
acetate | pH 7.0, 20°C, mutant E97Q, produced in E. coli | Methanosarcina thermophila | |
2.7.2.1 | 4.3 | - |
ATP | pH 7.0, 20°C, mutant E385D, produced in E. coli | Methanosarcina thermophila | |
2.7.2.1 | 4.5 | - |
ATP | - |
Methanosarcina thermophila | |
2.7.2.1 | 6.9 | - |
ATP | pH 7.0, 20°C, mutant E385A, produced in E. coli | Methanosarcina thermophila | |
2.7.2.1 | 7.2 | - |
acetate | pH 7.0, 20°C, mutant E97D, produced in E. coli | Methanosarcina thermophila | |
2.7.2.1 | 15 | - |
acetate | pH 7.0, 20°C, mutant E334A, produced in E. coli | Methanosarcina thermophila | |
2.7.2.1 | 17 | - |
acetate | pH 7.0, 20°C, mutant E385C, produced in E. coli | Methanosarcina thermophila | |
2.7.2.1 | 19 | - |
acetate | pH 7.0, 20°C, unaltered enzyme, produced in E. coli | Methanosarcina thermophila | |
2.7.2.1 | 22 | - |
acetate | - |
Methanosarcina thermophila | |
2.7.2.1 | 26 | - |
acetate | pH 7.0, 20°C, mutant E32A, produced in E. coli | Methanosarcina thermophila | |
2.7.2.1 | 44 | - |
acetate | pH 7.0, 20°C, mutant E385Q, produced in E. coli | Methanosarcina thermophila | |
2.7.2.1 | 86 | - |
acetate | pH 7.0, 20°C, mutant E385A, produced in E. coli | Methanosarcina thermophila | |
2.7.2.1 | 337 | - |
acetate | pH 7.0, 20°C, mutant E385D, produced in E. coli | Methanosarcina thermophila |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.1 | ATP + acetate | Methanosarcina thermophila | - |
ADP + acetyl phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.2.1 | Methanosarcina thermophila | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 4 | - |
mutant E384A | Methanosarcina thermophila |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.1 | ATP + acetate | - |
Methanosarcina thermophila | ADP + acetyl phosphate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.2.1 | dimer | - |
Methanosarcina thermophila |