EC Number | General Stability | Organism |
---|---|---|
2.7.2.1 | all 4 substrates of the forward and reverse reaction protect the enzyme from inactivation by diethyldicarbonate | Methanosarcina thermophila |
2.7.2.1 | unaffected by repeated freezing and thawing | Methanosarcina thermophila |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.2.1 | 2.8 | - |
ATP | pH 7.3, 37°C | Methanosarcina thermophila | |
2.7.2.1 | 22 | - |
acetate | pH 7.3, 37°C | Methanosarcina thermophila |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.2.1 | cytoplasm | - |
Methanosarcina thermophila | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.2.1 | Ca2+ | - |
Methanosarcina thermophila | |
2.7.2.1 | Co2+ | - |
Methanosarcina thermophila | |
2.7.2.1 | Mn2+ | - |
Methanosarcina thermophila | |
2.7.2.1 | additional information | no activity with Cu2+, Ni2+ or Zn2+ | Methanosarcina thermophila |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 53000 | - |
2 * 53000, alpha2 homodimer | Methanosarcina thermophila |
2.7.2.1 | 94000 | - |
gel filtration | Methanosarcina thermophila |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.1 | ATP + acetate | Methanosarcina thermophila | function in the initial activation of acetate for conversion to methane and CO2 | ADP + acetyl phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.2.1 | Methanosarcina thermophila | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
2.7.2.1 | stable to O2 | Methanosarcina thermophila |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.2.1 | - |
Methanosarcina thermophila |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 656 | - |
- |
Methanosarcina thermophila |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.2.1 | -196°C, stable for at least 4 months in liquid nitrogen | Methanosarcina thermophila |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.1 | ATP + acetate | - |
Methanosarcina thermophila | ADP + acetyl phosphate | - |
r | |
2.7.2.1 | ATP + acetate | function in the initial activation of acetate for conversion to methane and CO2 | Methanosarcina thermophila | ADP + acetyl phosphate | - |
r | |
2.7.2.1 | ATP + propionate | - |
Methanosarcina thermophila | ADP + propionyl phosphate | - |
r | |
2.7.2.1 | CTP + acetate | - |
Methanosarcina thermophila | CDP + acetyl phosphate | - |
r | |
2.7.2.1 | GTP + acetate | - |
Methanosarcina thermophila | GDP + acetyl phosphate | - |
r | |
2.7.2.1 | ITP + acetate | - |
Methanosarcina thermophila | IDP + acetyl phosphate | - |
r | |
2.7.2.1 | additional information | formate is no substrate | Methanosarcina thermophila | ? | - |
? | |
2.7.2.1 | TTP + acetate | - |
Methanosarcina thermophila | TDP + acetyl phosphate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.2.1 | dimer | 2 * 53000, alpha2 homodimer | Methanosarcina thermophila |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 30 | 70 | - |
Methanosarcina thermophila |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 70 | - |
stable to heating for 15 min, but rapidly inactivated at higher temperatures | Methanosarcina thermophila |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 7 | 7.4 | - |
Methanosarcina thermophila |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 5.5 | 9 | about 35% of maximum activity at pH 5.5 and 9.0 | Methanosarcina thermophila |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.7.2.1 | Methanosarcina thermophila | - |
4.8 | 4.7 |