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Literature summary extracted from

  • Boulatnikov, I.; Popov, K.M.
    Formation of functional heterodimers by isozymes 1 and 2 of pyruvate dehydrogenase kinase (2003), Biochim. Biophys. Acta, 1645, 183-192.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.2 coexpression of T7-tagged PDK1 and His-tagged PDK2 polypeptides in Escherichia coli, formation of a hybrid dimer Rattus norvegicus
2.7.11.2 expression of isozyme PDK2 as T7-tagged protein in Escherichia coli Rattus norvegicus
2.7.11.2 expression of isozyme PDK2 in Escherichia coli as His-tagged enzyme Rattus norvegicus
2.7.11.2 expression of T7-tagged and His-tagged isozyme PDK1 in Escherichia coli Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.2 ADP recombinant homodimers of PDK1 and PDK2 and heterodimers of PDK1 + PDK2, synergism with dichloroacetate; recombinant homodimers of PDK1 and PDK2 and heterodimers of PDK1 + PDK2, synergism with dichloroacetate Rattus norvegicus
2.7.11.2 Dichloroacetate recombinant homodimers of PDK1 and PDK2 and heterodimers of PDK1 + PDK2, synergism with dichloroacetate; recombinant homodimers of PDK1 and PDK2 and heterodimers of PDK1 + PDK2, synergism with dichloroacetate Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.2 0.007
-
 ATP recombinant His-tagged isozyme PDK2, 37°C, pH 7.8 Rattus norvegicus
2.7.11.2 0.034
-
 ATP recombinant His-tagged isozyme PDK1, 37°C, pH 7.8 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.2 mitochondrion
-
 Rattus norvegicus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.2 Mg2+
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.2 92000
-
 recombinant hybrid dimer of PDK1 and PDK2, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] Rattus norvegicus catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.2 Rattus norvegicus
-
 isozyme PDK2
-
2.7.11.2 Rattus norvegicus Q63065 isozyme PDK1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.2 recombinant from Escherichia coli as His-tagged isozyme PDK1 Rattus norvegicus
2.7.11.2 recombinant His-tagged PDK2 expressed in Escherichia coli Rattus norvegicus
2.7.11.2 recombinant hybrid dimer of T7-tagged PDK1 and His-tagged PDK2 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] recombinant hybrid enzyme of PDK1 and PDK2 phosphorylates site 3 with lower activity than the PDK1 homodimer Rattus norvegicus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] serine phosphorylation site 3 of subunit E1 is exclusively phosphorylated by isozyme PDK1, not by PDK2, which prefers site 1 over site 2 Rattus norvegicus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 Rattus norvegicus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
2.7.11.2 additional information binding of homodimers of PDK1 and PDK2, respectively and the heterodimer of PDK1+PDK2 to the pyruvate dehydrogenase complex via dihydrolipoyl transacetylase Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.2 PDK
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.2 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.2 7.8
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.2 ATP dependent on Rattus norvegicus