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Literature summary extracted from

  • Roche, T.E.; Reed, L.J.
    Monovalent cation requirement for ADP inhibition of pyruvate dehydrogenase kinase (1974), Biochem. Biophys. Res. Commun., 59, 1341-1348.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.2 ADP competitive to ATP; inhibition only in the presence of monovalent cations; Mg2+ does not protect Bos taurus
2.7.11.2 ATP above 0.5 mM, substrate inhibition, only in the presence of K+, Mg2+ does not protect Bos taurus
2.7.11.2 K+ synergism with ADP Bos taurus
2.7.11.2 Na+ above 50 mM, alone and synergism with ADP Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.2 additional information
-
 additional information the Km value for ADP depends on the presence and concentration of K+, effect of K+ on kinetic parameters Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.2 K+ inhibits in presence of ADP Bos taurus
2.7.11.2 Mg2+ requirement Bos taurus
2.7.11.2 Mg2+ actual substrate: MgATP2- Bos taurus
2.7.11.2 NH4+ activation in the absence of ADP, inhibits in presence of ADP Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] Bos taurus catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.2 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.2 kidney
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)]
-
 Bos taurus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 Bos taurus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.2 30
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.2 ATP dependent on Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.11.2 additional information
-
 additional information Ki value for ADP depends on the presence and concentration of monovalent cations, e.g. NH4+ or K+ Bos taurus