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Literature summary extracted from

  • Hucho, F.; Randall, D.D.; Roche, T.E.; Burgett, M.W.; Pelley, J.W.; Reed, L.J.
    alpha-Keto acid dehydrogenase complexes. XVII. Kinetic and regulatory properties of pyruvate dehydrogenase kinase and pyruvate dehydrogenase phosphatase from bovine kidney and heart (1972), Arch. Biochem. Biophys., 151, 328-340.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.2 dihydrolipoyl transacetylase
-
 Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.2 2-oxobutyrate
-
 Bos taurus
2.7.11.2 ADP competitive to ATP Bos taurus
2.7.11.2 CaCl2 no inhibition Bos taurus
2.7.11.2 additional information no inhibition by 2-oxoglutarate; no inhibition by cAMP Bos taurus
2.7.11.2 pyruvate synergism with ADP Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.2 0.0006
-
 [pyruvate dehydrogenase (acetyl-transferring)] pH 7.5, 30°C Bos taurus
2.7.11.2 0.0006
-
 [pyruvate dehydrogenase (acetyl-transferring)] in presence of dihydrolipoyl transacetylase Bos taurus
2.7.11.2 0.02
-
 ATP kidney enzyme Bos taurus
2.7.11.2 0.02
-
 Mg2+ kidney enzyme Bos taurus
2.7.11.2 0.02
-
 ATP pH 7.5, 30°C Bos taurus
2.7.11.2 0.02
-
 Mg2+ pH 7.5, 30°C Bos taurus
2.7.11.2 0.02
-
 [pyruvate dehydrogenase (acetyl-transferring)] pH 7.5, 30°C Bos taurus
2.7.11.2 0.02
-
 [pyruvate dehydrogenase (acetyl-transferring)] in absence of dihydrolipoyl transacetylase Bos taurus
2.7.11.2 0.02
-
 ATP pyruvate dehydrogenase complex Bos taurus
2.7.11.2 0.02
-
 Mg2+ pyruvate dehydrogenase complex Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.2 mitochondrion
-
 Bos taurus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.2 Mg2+ requirement Bos taurus
2.7.11.2 Mg2+ actual substrate: MgATP2- Bos taurus
2.7.11.2 Mn2+ requirement Bos taurus
2.7.11.2 Mn2+ can replace Mg2+ to some extent Bos taurus
2.7.11.2 additional information no activation by Ca2+ Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] Bos taurus regulatory role ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] Bos taurus catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.2 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.2 heart
-
 Bos taurus
-
2.7.11.2 kidney
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.2 ATP + casein kidney enzyme, low activity Bos taurus ADP + casein phosphate
-
 ?
2.7.11.2 ATP + [pyruvate dehydrogenase (acetyl-transferring)]
-
 Bos taurus ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate
-
 ?
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)]
-
 Bos taurus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] regulatory role Bos taurus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 Bos taurus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
2.7.11.2 additional information no activity with histones of calf thymus type II-A Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.2 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.2 7 7.2 in presence of Mg2+ or Mn2+ Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.2 5.5 8.5 about 50% or 60% of maximal activity at pH 5.5 and about 65% or 50% of maximal activity at pH 8.5, in the presence of Mg2+ or Mn2+, respectively Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.2 ATP dependent on Bos taurus
2.7.11.2 additional information no activation by cAMP Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.11.2 0.1
-
 ADP pH 7.5, 30°C Bos taurus