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Literature summary extracted from

  • Tripodi, K.E.J.; Podesta, F.E.
    Purification and structural and kinetic characterization of the pyrophosphate:fructose-6-phosphate 1-phosphotransferase from the Crassulacean acid metabolism plant, pineapple (1997), Plant Physiol., 113, 779-786.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.90 D-fructose 2,6-bisphosphate pH-dependent activation Ananas comosus
2.7.1.90 D-fructose 2,6-bisphosphate only weak activation in the reverse reaction Ananas comosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.90 additional information
-
 additional information kinetics Ananas comosus
2.7.1.90 0.011
-
 diphosphate pH 7.7, 25°C, in presence of fructose 2,6-bisphosphate Ananas comosus
2.7.1.90 0.094
-
 D-fructose 1,6-bisphosphate pH 7.7, 25°C, in presence of fructose 2,6-bisphosphate Ananas comosus
2.7.1.90 0.15
-
 phosphate pH 7.7, 25°C, in presence of fructose 2,6-bisphosphate Ananas comosus
2.7.1.90 0.63
-
 Mg2+ pH 7.7, 25°C Ananas comosus
2.7.1.90 0.89
-
 D-fructose 6-phosphate pH 7.7, 25°C, in presence of fructose 2,6-bisphosphate Ananas comosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.90 Mg2+ requirement Ananas comosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.90 61500
-
 2 * 61500, SDS-PAGE Ananas comosus
2.7.1.90 97000
-
 gel filtration Ananas comosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate Ananas comosus regulatory role in glucose metabolism phosphate + D-fructose 1,6-bisphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.90 Ananas comosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.90 40fold, to homogeneity Ananas comosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.90 leaf
-
 Ananas comosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.90 8.9
-
 reverse reaction, purified enzyme, pH 7.7, in presence of fructose 2,6-bisphosphate Ananas comosus
2.7.1.90 43.8
-
 forward reaction, purified enzyme, pH 7.7, in presence of fructose 2,6-bisphosphate Ananas comosus

Storage Stability

EC Number Storage Stability Organism
2.7.1.90 -80°C, purified enzyme, 25 mM Tris-HCl, pH 7.0, 20% glycerol v/v, 2 mM 2-mercaptoethanol, stable for at least 6 months Ananas comosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate substrate Mg-diphosphate Ananas comosus phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Ananas comosus phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate regulatory role in glucose metabolism Ananas comosus phosphate + D-fructose 1,6-bisphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.1.90 dimer 2 * 61500, SDS-PAGE Ananas comosus
2.7.1.90 More dimer-monomer mixture might exist Ananas comosus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.90 PFP
-
 Ananas comosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.90 additional information
-
 temperature-independent Ananas comosus
2.7.1.90 25
-
 assay at Ananas comosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.90 6.7 8.7 reverse reaction, pH-independent, broad optimum Ananas comosus
2.7.1.90 7.7 7.9 forward reaction, strongly pH-dependent Ananas comosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.90 Ananas comosus
-
-
 6.25