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Literature summary extracted from

  • Botha, F.C.; Small, J.G.C.; Burger, A.L.
    Characterization of pyrophosphate dependent phosphofructokinase from germinating bean seeds (1987), Plant Sci., 51, 151-157.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.90 D-fructose 2,6-bisphosphate kinetics Phaseolus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.90 D-fructose 6-phosphate
-
 Phaseolus vulgaris
2.7.1.90 diphosphate
-
 Phaseolus vulgaris
2.7.1.90 phosphate
-
 Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.90 additional information
-
 additional information kinetics Phaseolus vulgaris
2.7.1.90 0.1
-
 Mg2+ pH 7.5, 25°C Phaseolus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.90 Co2+
-
 Phaseolus vulgaris
2.7.1.90 divalent cations required Phaseolus vulgaris
2.7.1.90 divalent cations desecending order Mn2+, Mg2+, Co2+ Phaseolus vulgaris
2.7.1.90 divalent cations descending order Mg2+, Mn2+ and Co2+ Phaseolus vulgaris
2.7.1.90 Mg2+ requirement Phaseolus vulgaris
2.7.1.90 Mn2+ requirement Phaseolus vulgaris
2.7.1.90 additional information no activation by Cu2+ Phaseolus vulgaris
2.7.1.90 additional information no activation by Zn2+ Phaseolus vulgaris
2.7.1.90 additional information no activation by Fe2+ Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate Phaseolus vulgaris
-
 phosphate + D-fructose 1,6-bisphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.90 Phaseolus vulgaris
-
 cv. Top Crop
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.90 partial Phaseolus vulgaris

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.90 diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate mechanism Phaseolus vulgaris
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.90 seed germinating Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.90 6.5
-
 partially purified enzyme Phaseolus vulgaris

Storage Stability

EC Number Storage Stability Organism
2.7.1.90 -20°C, in 10 mM HEPES-NaOH buffer, pH 7.5, 10 mM 2-mercaptoethanol, 30% v/v glycerol, several months Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Phaseolus vulgaris phosphate + D-fructose 1,6-bisphosphate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.90 25
-
 assay at Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.90 8
-
-
 Phaseolus vulgaris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.90 additional information
-
 additional information
-
 Phaseolus vulgaris