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Literature summary extracted from

  • Lillehaug, J.R.
    Physicochemical properties of T4 polynucleotide kinase (1977), Eur. J. Biochem., 73, 499-506.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.78 spermine stabilizes the enzyme in an oligomeric state Tequatrovirus T4

Application

EC Number Application Comment Organism
2.7.1.78 biotechnology application in DNA and RNA sequencing Tequatrovirus T4
2.7.1.78 synthesis enzyme is an important tool in the synthesis of genes corresponding to yeast alanine tRNAand to precursor tyrosine tRNA of Escherichia coli Tequatrovirus T4

General Stability

EC Number General Stability Organism
2.7.1.78 high ionic strength, 0.1 M KCl, spermine, ATP and thymidine 3'-monophosphate stabilize the oligomeric structure Tequatrovirus T4
2.7.1.78 high ionic strength, e.g. 0.1 M KCl, spermine, ATP and thymidine 3'-monophosphate are essential to stabilize the enzyme in an oligomeric state Tequatrovirus T4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.78 33000
-
 4 * 33000, sedimentation analysis and amino acid sequence determination Tequatrovirus T4

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Tequatrovirus T4
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA
-
 Tequatrovirus T4 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + deoxynucleoside 3'-phosphate
-
 Tequatrovirus T4 ADP + deoxynucleoside 3',5'-diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.78 More each monomer contains 2 SH-groups, one of which is more buried Tequatrovirus T4
2.7.1.78 More high ionic strength, e.g. 0.1 M KCl, spermine, ATP and thymidine 3'-monophosphate are essential to stabilize the enzyme in an oligomeric state Tequatrovirus T4
2.7.1.78 tetramer 4 * 33000, sedimentation analysis and amino acid sequence determination Tequatrovirus T4

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.78 ATP dependent on Tequatrovirus T4
2.7.1.78 ATP stabilizes the enzyme in an oligomeric state Tequatrovirus T4