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Literature summary extracted from

  • Guranowski, A.
    Nucleoside phosphotransferase from yellow lupin seedling cotyledons (1979), Biochim. Biophys. Acta, 569, 13-22.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.77 the enzyme tolerates repeated freeze-thawing Lupinus luteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.77 0.4
-
 Inosine pH 8.0, 30°C Lupinus luteus
2.7.1.77 0.4
-
 5'-AMP pH 8.0, 30°C Lupinus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.77 72000
-
 gel filtration Lupinus luteus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.77 Lupinus luteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.77
-
 Lupinus luteus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.77 cotyledon
-
 Lupinus luteus
-
2.7.1.77 seedling cotyledon Lupinus luteus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.77 1.1
-
-
 Lupinus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.77 3'-AMP + adenosine
-
 Lupinus luteus AMP + adenosine
-
 ?
2.7.1.77 3'-AMP + cytidine
-
 Lupinus luteus CMP + adenosine
-
 ?
2.7.1.77 3'-AMP + guanosine
-
 Lupinus luteus GMP + adenosine
-
 ?
2.7.1.77 3'-AMP + inosine
-
 Lupinus luteus IMP + adenosine
-
 ?
2.7.1.77 3'-AMP + uridine
-
 Lupinus luteus UMP + adenosine
-
 ?
2.7.1.77 4-nitrophenyl phosphate + adenosine
-
 Lupinus luteus 5'-AMP + 4-nitrophenol
-
 ?
2.7.1.77 4-nitrophenyl phosphate + inosine
-
 Lupinus luteus 4-nitrophenol + IMP
-
 ?
2.7.1.77 4-nitrophenyl phosphate + uridine
-
 Lupinus luteus 4-nitrophenol + 5'-UMP
-
 ?
2.7.1.77 5'-AMP + adenosine
-
 Lupinus luteus AMP + adenosine
-
 ?
2.7.1.77 5'-AMP + cytidine
-
 Lupinus luteus CMP + adenosine
-
 ?
2.7.1.77 5'-AMP + guanosine
-
 Lupinus luteus GMP + adenosine
-
 ?
2.7.1.77 5'-AMP + inosine
-
 Lupinus luteus IMP + adenosine
-
 ?
2.7.1.77 5'-AMP + uridine
-
 Lupinus luteus UMP + adenosine
-
 ?
2.7.1.77 5'-CMP + adenosine
-
 Lupinus luteus AMP + cytidine
-
 ?
2.7.1.77 5'-CMP + cytidine
-
 Lupinus luteus CMP + cytidine
-
 ?
2.7.1.77 5'-CMP + guanosine
-
 Lupinus luteus GMP + cytidine
-
 ?
2.7.1.77 5'-CMP + inosine
-
 Lupinus luteus IMP + cytidine
-
 ?
2.7.1.77 5'-CMP + uridine
-
 Lupinus luteus UMP + cytidine
-
 ?
2.7.1.77 5'-GMP + adenosine
-
 Lupinus luteus AMP + guanosine
-
 ?
2.7.1.77 5'-GMP + cytidine
-
 Lupinus luteus CMP + guanosine
-
 ?
2.7.1.77 5'-GMP + inosine
-
 Lupinus luteus IMP + guanosine
-
 ?
2.7.1.77 5'-GMP + uridine
-
 Lupinus luteus UMP + guanosine
-
 ?
2.7.1.77 5'-IMP + adenosine
-
 Lupinus luteus AMP + inosine
-
 ?
2.7.1.77 5'-IMP + cytidine
-
 Lupinus luteus CMP + inosine
-
 ?
2.7.1.77 5'-IMP + guanosine
-
 Lupinus luteus GMP + inosine
-
 ?
2.7.1.77 5'-IMP + inosine
-
 Lupinus luteus IMP + inosine
-
 ?
2.7.1.77 5'-IMP + uridine
-
 Lupinus luteus UMP + inosine
-
 ?
2.7.1.77 5'-UMP + adenosine
-
 Lupinus luteus AMP + uridine
-
 ?
2.7.1.77 5'-UMP + cytidine
-
 Lupinus luteus CMP + uridine
-
 ?
2.7.1.77 5'-UMP + guanosine
-
 Lupinus luteus GMP + uridine
-
 ?
2.7.1.77 5'-UMP + inosine
-
 Lupinus luteus IMP + uridine
-
 ?
2.7.1.77 5'-UMP + uridine
-
 Lupinus luteus UMP + uridine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.77 monomer 1 * 72000, SDS-PAGE Lupinus luteus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.77 63
-
 pH 8.0, 3 min, 50 mM Tris-HCl buffer, 50% loss of activity Lupinus luteus
2.7.1.77 68
-
 pH 8.0, 3 min, 50 mM Tris-HCl buffer containing 10% glycerol, 50% loss of activity Lupinus luteus
2.7.1.77 72
-
 pH 8.0, 3 min, 50 mM potassium phosphate buffer containing 10% glycerol, pH 6.8, 50% loss of activity Lupinus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.77 8
-
-
 Lupinus luteus