BRENDA - Enzyme Database show

Novel deoxynucleoside-phosphorylating enzymes in mycoplasmas: evidence for efficient utilization of deoxynucleosides

Wang, L.; Westberg, J.; Bolske, G.; Eriksson, S.; Mol. Microbiol. 42, 1065-1073 (2001)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.76
expression in Escherichia coli
Mycoplasma mycoides
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.76
0.011
-
ATP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
0.012
-
deoxyadenosine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
0.021
-
deoxyguanosine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
0.022
-
CTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
0.063
-
TTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
0.18
-
deoxycytidine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
0.18
-
UTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.76
27000
-
2 * 27000, His-tagged enzyme, SDS-PAGE
Mycoplasma mycoides
2.7.1.76
50000
-
gel filtration
Mycoplasma mycoides
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.76
Acholeplasma laidlawii
-
-
-
2.7.1.76
Mycoplasma arginini
-
-
-
2.7.1.76
Mycoplasma mycoides
-
subspecies mycoides SC
-
2.7.1.76
Mycoplasma pneumoniae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.76
recombinant His-tagged enzyme, metal affinity chromatography
Mycoplasma mycoides
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.76
ATP + deoxyadenosine
-
641988
Acholeplasma laidlawii
ADP + dAMP
-
641988
Acholeplasma laidlawii
?
2.7.1.76
ATP + deoxyadenosine
-
641988
Mycoplasma mycoides
ADP + dAMP
-
641988
Mycoplasma mycoides
?
2.7.1.76
ATP + deoxyadenosine
-
641988
Mycoplasma pneumoniae
ADP + dAMP
-
641988
Mycoplasma pneumoniae
?
2.7.1.76
ATP + deoxyadenosine
-
641988
Mycoplasma arginini
ADP + dAMP
-
641988
Mycoplasma arginini
?
2.7.1.76
ATP + deoxycytidine
-
641988
Mycoplasma mycoides
ADP + dCMP
-
641988
Mycoplasma mycoides
?
2.7.1.76
ATP + deoxyguanosine
approx. 40% of activity with deoxyadenosine
641988
Mycoplasma mycoides
ADP + dGMP
-
641988
Mycoplasma mycoides
?
2.7.1.76
CTP + deoxyadenosine
-
641988
Mycoplasma mycoides
CDP + dAMP
-
641988
Mycoplasma mycoides
?
2.7.1.76
GTP + deoxyadenosine
10% of activity with ATP
641988
Mycoplasma mycoides
GDP + dAMP
-
641988
Mycoplasma mycoides
?
2.7.1.76
TTP + deoxyadenosine
-
641988
Mycoplasma mycoides
TDP + dAMP
-
641988
Mycoplasma mycoides
?
2.7.1.76
UTP + deoxyadenosine
-
641988
Mycoplasma mycoides
UDP + dAMP
-
641988
Mycoplasma mycoides
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.76
dimer
2 * 27000, His-tagged enzyme, SDS-PAGE
Mycoplasma mycoides
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.76
0.36
-
UTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
0.61
-
deoxyguanosine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
0.62
-
CTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
0.84
-
ATP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
1.23
-
deoxycytidine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
1.48
-
deoxyadenosine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
1.64
-
TTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.76
expression in Escherichia coli
Mycoplasma mycoides
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.76
0.011
-
ATP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
0.012
-
deoxyadenosine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
0.021
-
deoxyguanosine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
0.022
-
CTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
0.063
-
TTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
0.18
-
deoxycytidine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
0.18
-
UTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.76
27000
-
2 * 27000, His-tagged enzyme, SDS-PAGE
Mycoplasma mycoides
2.7.1.76
50000
-
gel filtration
Mycoplasma mycoides
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.76
recombinant His-tagged enzyme, metal affinity chromatography
Mycoplasma mycoides
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.76
ATP + deoxyadenosine
-
641988
Acholeplasma laidlawii
ADP + dAMP
-
641988
Acholeplasma laidlawii
?
2.7.1.76
ATP + deoxyadenosine
-
641988
Mycoplasma mycoides
ADP + dAMP
-
641988
Mycoplasma mycoides
?
2.7.1.76
ATP + deoxyadenosine
-
641988
Mycoplasma pneumoniae
ADP + dAMP
-
641988
Mycoplasma pneumoniae
?
2.7.1.76
ATP + deoxyadenosine
-
641988
Mycoplasma arginini
ADP + dAMP
-
641988
Mycoplasma arginini
?
2.7.1.76
ATP + deoxycytidine
-
641988
Mycoplasma mycoides
ADP + dCMP
-
641988
Mycoplasma mycoides
?
2.7.1.76
ATP + deoxyguanosine
approx. 40% of activity with deoxyadenosine
641988
Mycoplasma mycoides
ADP + dGMP
-
641988
Mycoplasma mycoides
?
2.7.1.76
CTP + deoxyadenosine
-
641988
Mycoplasma mycoides
CDP + dAMP
-
641988
Mycoplasma mycoides
?
2.7.1.76
GTP + deoxyadenosine
10% of activity with ATP
641988
Mycoplasma mycoides
GDP + dAMP
-
641988
Mycoplasma mycoides
?
2.7.1.76
TTP + deoxyadenosine
-
641988
Mycoplasma mycoides
TDP + dAMP
-
641988
Mycoplasma mycoides
?
2.7.1.76
UTP + deoxyadenosine
-
641988
Mycoplasma mycoides
UDP + dAMP
-
641988
Mycoplasma mycoides
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.76
dimer
2 * 27000, His-tagged enzyme, SDS-PAGE
Mycoplasma mycoides
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.76
0.36
-
UTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
0.61
-
deoxyguanosine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
0.62
-
CTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
0.84
-
ATP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
1.23
-
deoxycytidine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
1.48
-
deoxyadenosine
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides
2.7.1.76
1.64
-
TTP
pH 7.6, 37°C, recombinant enzyme
Mycoplasma mycoides