BRENDA - Enzyme Database show

Phosphorylation of 4'-thio-beta-D-arabinofuranosylcytosine and its analogs by human deoxycytidine kinase

Someya, H.; Shaddix, S.C.; Tiwari, K.N.; Secrist, J.A., 3rd; Parker, W.B.; J. Pharmacol. Exp. Ther. 304, 1314-1322 (2003)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
4'-thio-beta-D-arabinofuranosylcytosine
IC50 0.000024 mM
Homo sapiens
2.7.1.74
5-chloro-4'-thio-beta-D-arabinofuranosylcytosine
IC50 0.051 mM
Homo sapiens
2.7.1.74
5-fluoro-4'-thio-beta-D-arabinofuranosylcytosine
IC50 0.0000020 mM
Homo sapiens
2.7.1.74
cytosine arabinoside
IC50 0.0000055 mM
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.00013
-
deoxycytidine
cosubstrate UTP 37°C, pH 8.0
Homo sapiens
2.7.1.74
0.00032
-
4'-thio-2'-deoxycytidine
cosubstrate ATP 37°C, pH 8.0
Homo sapiens
2.7.1.74
0.00041
-
beta-D-arabinofuranosylcytosine
cosubstrate UTP 37°C, pH 8.0
Homo sapiens
2.7.1.74
0.00057
-
deoxycytidine
cosubstrate ATP 37°C, pH 8.0
Homo sapiens
2.7.1.74
0.015
-
beta-D-arabinofuranosylcytosine
cosubstrate ATP 37°C, pH 8.0
Homo sapiens
2.7.1.74
0.02
-
4'-thio-beta-D-arabinofuranosylcytosine
cosubstrate UTP 37°C, pH 8.0
Homo sapiens
2.7.1.74
0.088
-
4'-thio-beta-D-arabinofuranosylcytosine
cosubstrate ATP 37°C, pH 8.0
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
UTP + deoxycytidine
Homo sapiens
UTP is physiological phosphate donor
UDP + dCMP
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
4'-thio-2'-deoxycytidine + UTP
-
641970
Homo sapiens
UDP + 4'-thio-2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
4'-thio-beta-D-arabinofuranosylcytosine + UTP
-
641970
Homo sapiens
UDP + 4'-thio-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + cytosine arabinoside
-
641970
Homo sapiens
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
641970
Homo sapiens
NDP + dCMP
-
-
-
?
2.7.1.74
UTP + deoxycytidine
UTP is physiological phosphate donor
641970
Homo sapiens
UDP + dCMP
-
-
-
?
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.1.74
0.000002
-
IC50 0.0000020 mM
Homo sapiens
5-fluoro-4'-thio-beta-D-arabinofuranosylcytosine
2.7.1.74
0.0000055
-
IC50 0.0000055 mM
Homo sapiens
cytosine arabinoside
2.7.1.74
0.000024
-
IC50 0.000024 mM
Homo sapiens
4'-thio-beta-D-arabinofuranosylcytosine
2.7.1.74
0.051
-
IC50 0.051 mM
Homo sapiens
5-chloro-4'-thio-beta-D-arabinofuranosylcytosine
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.1.74
0.000002
-
IC50 0.0000020 mM
Homo sapiens
5-fluoro-4'-thio-beta-D-arabinofuranosylcytosine
2.7.1.74
0.0000055
-
IC50 0.0000055 mM
Homo sapiens
cytosine arabinoside
2.7.1.74
0.000024
-
IC50 0.000024 mM
Homo sapiens
4'-thio-beta-D-arabinofuranosylcytosine
2.7.1.74
0.051
-
IC50 0.051 mM
Homo sapiens
5-chloro-4'-thio-beta-D-arabinofuranosylcytosine
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
4'-thio-beta-D-arabinofuranosylcytosine
IC50 0.000024 mM
Homo sapiens
2.7.1.74
5-chloro-4'-thio-beta-D-arabinofuranosylcytosine
IC50 0.051 mM
Homo sapiens
2.7.1.74
5-fluoro-4'-thio-beta-D-arabinofuranosylcytosine
IC50 0.0000020 mM
Homo sapiens
2.7.1.74
cytosine arabinoside
IC50 0.0000055 mM
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.00013
-
deoxycytidine
cosubstrate UTP 37°C, pH 8.0
Homo sapiens
2.7.1.74
0.00032
-
4'-thio-2'-deoxycytidine
cosubstrate ATP 37°C, pH 8.0
Homo sapiens
2.7.1.74
0.00041
-
beta-D-arabinofuranosylcytosine
cosubstrate UTP 37°C, pH 8.0
Homo sapiens
2.7.1.74
0.00057
-
deoxycytidine
cosubstrate ATP 37°C, pH 8.0
Homo sapiens
2.7.1.74
0.015
-
beta-D-arabinofuranosylcytosine
cosubstrate ATP 37°C, pH 8.0
Homo sapiens
2.7.1.74
0.02
-
4'-thio-beta-D-arabinofuranosylcytosine
cosubstrate UTP 37°C, pH 8.0
Homo sapiens
2.7.1.74
0.088
-
4'-thio-beta-D-arabinofuranosylcytosine
cosubstrate ATP 37°C, pH 8.0
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
UTP + deoxycytidine
Homo sapiens
UTP is physiological phosphate donor
UDP + dCMP
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
4'-thio-2'-deoxycytidine + UTP
-
641970
Homo sapiens
UDP + 4'-thio-2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
4'-thio-beta-D-arabinofuranosylcytosine + UTP
-
641970
Homo sapiens
UDP + 4'-thio-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + cytosine arabinoside
-
641970
Homo sapiens
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
641970
Homo sapiens
NDP + dCMP
-
-
-
?
2.7.1.74
UTP + deoxycytidine
UTP is physiological phosphate donor
641970
Homo sapiens
UDP + dCMP
-
-
-
?