BRENDA - Enzyme Database show

Differences in kinetic properties of pure recombinant human and mouse deoxycytidine kinase

Johansson, M.; Karlsson, A.; Biochem. Pharmacol. 50, 163-168 (1995)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.74
-
Homo sapiens
2.7.1.74
-
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
dCTP
at 0.013 mM, substrates ATP, deoxycytosine, 30% inhibition
Homo sapiens
2.7.1.74
dCTP
at 0.013 mM, substrates ATP, deoxycytosine, 50% inhibition
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.00005
-
2'-deoxycytidine
cosubstrate UTP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
0.0001
-
2'-deoxycytidine
cosubstrate UTP, 37°C, pH 7.6
Mus musculus
2.7.1.74
0.00016
-
2'-deoxycytidine
cosubstrate ATP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
0.00018
-
2'-deoxycytidine
cosubstrate ATP, 37°C, pH 7.6
Mus musculus
2.7.1.74
0.00099
-
2-chloro-2'-deoxyadenosine
cosubstrate UTP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
0.0063
-
2-chloro-2'-deoxyadenosine
cosubstrate UTP, 37°C, pH 7.6
Mus musculus
2.7.1.74
0.017
-
2-chloro-2'-deoxyadenosine
cosubstrate ATP, 37°C, pH 7.6
Mus musculus
2.7.1.74
0.019
-
2'-deoxyadenosine
cosubstrate UTP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
0.024
-
2-chloro-2'-deoxyadenosine
cosubstrate ATP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
0.15
-
2',3'-dideoxycytidine
cosubstrate UTP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
0.15
-
2'-deoxyadenosine
cosubstrate UTP, 37°C, pH 7.6
Mus musculus
2.7.1.74
0.23
-
2',3'-dideoxycytidine
cosubstrate ATP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
0.48
-
2',3'-dideoxycytidine
cosubstrate UTP, 37°C, pH 7.6
Mus musculus
2.7.1.74
0.48
-
2'-deoxyadenosine
cosubstrate ATP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
0.55
-
2'-deoxyadenosine
cosubstrate ATP, 37°C, pH 7.6
Mus musculus
2.7.1.74
0.67
-
2',3'-dideoxycytidine
cosubstrate ATP, 37°C, pH 7.6
Mus musculus
2.7.1.74
2
-
9-beta-D-arabinofuranosylguanine
cosubstrate UTP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
3
-
9-beta-D-arabinofuranosylguanine
cosubstrate ATP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
3
-
9-beta-D-arabinofuranosylguanine
cosubstrate UTP, 37°C, pH 7.6
Mus musculus
2.7.1.74
5
-
9-beta-D-arabinofuranosylguanine
cosubstrate ATP, 37°C, pH 7.6
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
-
-
-
2.7.1.74
Mus musculus
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
2-chloro-2'-deoxyadenosine + NTP
-
641953
Mus musculus
NDP + 2-chloro-2'-deoxy-AMP
-
-
-
?
2.7.1.74
2-chloro-2'-deoxyadenosine + NTP
-
641953
Homo sapiens
NDP + 2-chloro-2'-deoxy-AMP
-
-
-
?
2.7.1.74
9-beta-D-arabinofuranosylguanine + NTP
-
641953
Mus musculus
NDP + 9-beta-D-arabinofuranosylguanine 5'-phosphate
-
-
-
?
2.7.1.74
9-beta-D-arabinofuranosylguanine + NTP
-
641953
Homo sapiens
NDP + 9-beta-D-arabinofuranosylguanine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + 2',3'-dideoxycytidine
-
641953
Mus musculus
NDP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + 2',3'-dideoxycytidine
-
641953
Homo sapiens
NDP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
-
641953
Mus musculus
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
-
641953
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
641953
Mus musculus
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
641953
Homo sapiens
NDP + dCMP
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
-
Homo sapiens
2.7.1.74
-
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
dCTP
at 0.013 mM, substrates ATP, deoxycytosine, 30% inhibition
Homo sapiens
2.7.1.74
dCTP
at 0.013 mM, substrates ATP, deoxycytosine, 50% inhibition
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.00005
-
2'-deoxycytidine
cosubstrate UTP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
0.0001
-
2'-deoxycytidine
cosubstrate UTP, 37°C, pH 7.6
Mus musculus
2.7.1.74
0.00016
-
2'-deoxycytidine
cosubstrate ATP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
0.00018
-
2'-deoxycytidine
cosubstrate ATP, 37°C, pH 7.6
Mus musculus
2.7.1.74
0.00099
-
2-chloro-2'-deoxyadenosine
cosubstrate UTP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
0.0063
-
2-chloro-2'-deoxyadenosine
cosubstrate UTP, 37°C, pH 7.6
Mus musculus
2.7.1.74
0.017
-
2-chloro-2'-deoxyadenosine
cosubstrate ATP, 37°C, pH 7.6
Mus musculus
2.7.1.74
0.019
-
2'-deoxyadenosine
cosubstrate UTP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
0.024
-
2-chloro-2'-deoxyadenosine
cosubstrate ATP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
0.15
-
2',3'-dideoxycytidine
cosubstrate UTP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
0.15
-
2'-deoxyadenosine
cosubstrate UTP, 37°C, pH 7.6
Mus musculus
2.7.1.74
0.23
-
2',3'-dideoxycytidine
cosubstrate ATP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
0.48
-
2',3'-dideoxycytidine
cosubstrate UTP, 37°C, pH 7.6
Mus musculus
2.7.1.74
0.48
-
2'-deoxyadenosine
cosubstrate ATP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
0.55
-
2'-deoxyadenosine
cosubstrate ATP, 37°C, pH 7.6
Mus musculus
2.7.1.74
0.67
-
2',3'-dideoxycytidine
cosubstrate ATP, 37°C, pH 7.6
Mus musculus
2.7.1.74
2
-
9-beta-D-arabinofuranosylguanine
cosubstrate UTP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
3
-
9-beta-D-arabinofuranosylguanine
cosubstrate ATP, 37°C, pH 7.6
Homo sapiens
2.7.1.74
3
-
9-beta-D-arabinofuranosylguanine
cosubstrate UTP, 37°C, pH 7.6
Mus musculus
2.7.1.74
5
-
9-beta-D-arabinofuranosylguanine
cosubstrate ATP, 37°C, pH 7.6
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
2-chloro-2'-deoxyadenosine + NTP
-
641953
Mus musculus
NDP + 2-chloro-2'-deoxy-AMP
-
-
-
?
2.7.1.74
2-chloro-2'-deoxyadenosine + NTP
-
641953
Homo sapiens
NDP + 2-chloro-2'-deoxy-AMP
-
-
-
?
2.7.1.74
9-beta-D-arabinofuranosylguanine + NTP
-
641953
Mus musculus
NDP + 9-beta-D-arabinofuranosylguanine 5'-phosphate
-
-
-
?
2.7.1.74
9-beta-D-arabinofuranosylguanine + NTP
-
641953
Homo sapiens
NDP + 9-beta-D-arabinofuranosylguanine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + 2',3'-dideoxycytidine
-
641953
Mus musculus
NDP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + 2',3'-dideoxycytidine
-
641953
Homo sapiens
NDP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
-
641953
Mus musculus
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
-
641953
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
641953
Mus musculus
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
641953
Homo sapiens
NDP + dCMP
-
-
-
?