BRENDA - Enzyme Database show

Human T-lymphoblast deoxycytidine kinase: purification and properties

Datta, N.S.; Shewach, D.S.; Hurley, M.C.; Mitchell, B.S.; Fox, I.H.; Biochemistry 28, 114-123 (1989)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.7.1.74
dithiothreitol, MgCl2, ATP, KCl and glycerol stabilize
Homo sapiens
2.7.1.74
very unstable at low protein concentrations
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
2-chloroadenosine
-
Homo sapiens
2.7.1.74
Ba2+
-
Homo sapiens
2.7.1.74
Cu2+
-
Homo sapiens
2.7.1.74
cytidine
deoxycytidine as substrate
Homo sapiens
2.7.1.74
cytosine arabinoside
deoxycytidine as substrate
Homo sapiens
2.7.1.74
deoxyadenosine
deoxycytidine
Homo sapiens
2.7.1.74
deoxyguanosine
deoxycytidine as substrate
Homo sapiens
2.7.1.74
Dideoxycytidine
-
Homo sapiens
2.7.1.74
HgCl2
-
Homo sapiens
2.7.1.74
additional information
little or no inhibition by AMP, dTMP, adenosine, guanosine; little or no inhibition by deoxyuridine; little or no inhibition by ribavirin; no inhibition by inosine, xanthosine, S-adenosylhomocysteine, S-adenosylmethionine; no inhibition by uridine, thymidine; overview
Homo sapiens
2.7.1.74
Nucleosides
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.0008
0.0015
deoxycytidine
37°C, pH 7.0
Homo sapiens
2.7.1.74
0.02
0.04
cytosine arabinoside
pH 7.0, 37°C
Homo sapiens
2.7.1.74
0.33
0.43
deoxyguanosine
37°C, pH 7.0
Homo sapiens
2.7.1.74
0.45
-
cytidine
37°C, pH 7.0
Homo sapiens
2.7.1.74
0.5
-
deoxyadenosine
37°C, pH 7.0
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Ca2+
activation
Homo sapiens
2.7.1.74
Co2+
about 30% as efficient as Mg2+; activation
Homo sapiens
2.7.1.74
Fe2+
about 60% as efficient as Mg2+; activation
Homo sapiens
2.7.1.74
Mg2+
-
Homo sapiens
2.7.1.74
Mn2+
about 90% as efficient as Mg2+; requirement
Homo sapiens
2.7.1.74
additional information
no activation by Ba2+, Cu2+; no activation by monovalent cations
Homo sapiens
2.7.1.74
Ni2+
not
Homo sapiens
2.7.1.74
Zn2+
about 30% as efficient as Mg2+; activation
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.74
30500
-
2 * 30500, SDS-PAGE
Homo sapiens
2.7.1.74
60000
-
gel filtration
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.74
affinity chromatography
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
leukemia cell
cell line MOLT-4; leukemic T-lymphoblasts
Homo sapiens
-
2.7.1.74
MOLT-4 cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
4
-
cytidine as substrate, 37°C, pH 7.0
Homo sapiens
2.7.1.74
7.2
-
deoxyguanosine as substrate, 37°C, pH 7.0
Homo sapiens
2.7.1.74
8
-
37°C, pH 7.0
Homo sapiens
2.7.1.74
13.5
-
deoxyadenosine as substrate, 37°C, pH 7.0
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.74
-85°C, in 1 mg/ml bovine serum albumin, 20% v/v glycerol, 0.2 M potassium phosphate, 2 mM ATP, 2.4 mM MgCl2 and 0.025 M DTT, more than 12 months
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641942
Homo sapiens
ADP + 5'-dCMP
-
-
-
?
2.7.1.74
ATP + cytidine
-
641942
Homo sapiens
ADP + ?
-
-
-
?
2.7.1.74
CTP + 2'-deoxycytidine
poor
641942
Homo sapiens
CDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dGTP + 2'-deoxycytidine
best phosphate donor
641942
Homo sapiens
dGDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
GTP + 2'-deoxycytidine
as good as ATP
641942
Homo sapiens
GDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
NTP + cytosine arabinoside
-
641942
Homo sapiens
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
-
641942
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
enzyme shows little specificity towards phosphate donor
641942
Homo sapiens
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
no substrate: nucleoside diphosphates and their deoxy derivatives
641942
Homo sapiens
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + deoxyguanosine
-
641942
Homo sapiens
NDP + dGMP
-
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
poor
641942
Homo sapiens
UDP + 2'-deoxy-CMP
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.74
dimer
2 * 30500, SDS-PAGE
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.74
7
-
broad
Homo sapiens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.74
5.8
10
about half-maximal activity at pH 5.8 and pH 10, about 90% of maximal activity at pH 6.5 and pH 9
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.1.74
dithiothreitol, MgCl2, ATP, KCl and glycerol stabilize
Homo sapiens
2.7.1.74
very unstable at low protein concentrations
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
2-chloroadenosine
-
Homo sapiens
2.7.1.74
Ba2+
-
Homo sapiens
2.7.1.74
Cu2+
-
Homo sapiens
2.7.1.74
cytidine
deoxycytidine as substrate
Homo sapiens
2.7.1.74
cytosine arabinoside
deoxycytidine as substrate
Homo sapiens
2.7.1.74
deoxyadenosine
deoxycytidine
Homo sapiens
2.7.1.74
deoxyguanosine
deoxycytidine as substrate
Homo sapiens
2.7.1.74
Dideoxycytidine
-
Homo sapiens
2.7.1.74
HgCl2
-
Homo sapiens
2.7.1.74
additional information
little or no inhibition by AMP, dTMP, adenosine, guanosine; little or no inhibition by deoxyuridine; little or no inhibition by ribavirin; no inhibition by inosine, xanthosine, S-adenosylhomocysteine, S-adenosylmethionine; no inhibition by uridine, thymidine; overview
Homo sapiens
2.7.1.74
Nucleosides
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.0008
0.0015
deoxycytidine
37°C, pH 7.0
Homo sapiens
2.7.1.74
0.02
0.04
cytosine arabinoside
pH 7.0, 37°C
Homo sapiens
2.7.1.74
0.33
0.43
deoxyguanosine
37°C, pH 7.0
Homo sapiens
2.7.1.74
0.45
-
cytidine
37°C, pH 7.0
Homo sapiens
2.7.1.74
0.5
-
deoxyadenosine
37°C, pH 7.0
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Ca2+
activation
Homo sapiens
2.7.1.74
Co2+
about 30% as efficient as Mg2+; activation
Homo sapiens
2.7.1.74
Fe2+
about 60% as efficient as Mg2+; activation
Homo sapiens
2.7.1.74
Mg2+
-
Homo sapiens
2.7.1.74
Mn2+
about 90% as efficient as Mg2+; requirement
Homo sapiens
2.7.1.74
additional information
no activation by Ba2+, Cu2+; no activation by monovalent cations
Homo sapiens
2.7.1.74
Ni2+
not
Homo sapiens
2.7.1.74
Zn2+
about 30% as efficient as Mg2+; activation
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.74
30500
-
2 * 30500, SDS-PAGE
Homo sapiens
2.7.1.74
60000
-
gel filtration
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
affinity chromatography
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
leukemia cell
cell line MOLT-4; leukemic T-lymphoblasts
Homo sapiens
-
2.7.1.74
MOLT-4 cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
4
-
cytidine as substrate, 37°C, pH 7.0
Homo sapiens
2.7.1.74
7.2
-
deoxyguanosine as substrate, 37°C, pH 7.0
Homo sapiens
2.7.1.74
8
-
37°C, pH 7.0
Homo sapiens
2.7.1.74
13.5
-
deoxyadenosine as substrate, 37°C, pH 7.0
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.74
-85°C, in 1 mg/ml bovine serum albumin, 20% v/v glycerol, 0.2 M potassium phosphate, 2 mM ATP, 2.4 mM MgCl2 and 0.025 M DTT, more than 12 months
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641942
Homo sapiens
ADP + 5'-dCMP
-
-
-
?
2.7.1.74
ATP + cytidine
-
641942
Homo sapiens
ADP + ?
-
-
-
?
2.7.1.74
CTP + 2'-deoxycytidine
poor
641942
Homo sapiens
CDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dGTP + 2'-deoxycytidine
best phosphate donor
641942
Homo sapiens
dGDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
GTP + 2'-deoxycytidine
as good as ATP
641942
Homo sapiens
GDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
NTP + cytosine arabinoside
-
641942
Homo sapiens
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
-
641942
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
enzyme shows little specificity towards phosphate donor
641942
Homo sapiens
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
no substrate: nucleoside diphosphates and their deoxy derivatives
641942
Homo sapiens
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + deoxyguanosine
-
641942
Homo sapiens
NDP + dGMP
-
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
poor
641942
Homo sapiens
UDP + 2'-deoxy-CMP
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.74
dimer
2 * 30500, SDS-PAGE
Homo sapiens
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.74
7
-
broad
Homo sapiens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.74
5.8
10
about half-maximal activity at pH 5.8 and pH 10, about 90% of maximal activity at pH 6.5 and pH 9
Homo sapiens