BRENDA - Enzyme Database show

Metabolism in deoxyribonucleotides. IV. Purification and properties of deoxycytidine kinase of calf thymus

Kozai, Y.; Sonoda, S.; Kobayashi, S.; Sugino, Y.; J. Biochem. 71, 485-496 (1972)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
ADP
-
Bos taurus
2.7.1.74
CDP
strong
Bos taurus
2.7.1.74
CTP
-
Bos taurus
2.7.1.74
cytidine
not
Bos taurus
2.7.1.74
dADP
strong
Bos taurus
2.7.1.74
dATP
strong
Bos taurus
2.7.1.74
dCDP
strong, ATP as substrate
Bos taurus
2.7.1.74
dCMP
ATP as substrate; product inhibition, strong
Bos taurus
2.7.1.74
dCTP
ATP as substrate
Bos taurus
2.7.1.74
deoxycytidine
cytidine as substrate; deoxyguanosine as substrate
Bos taurus
2.7.1.74
deoxyguanosine
not
Bos taurus
2.7.1.74
dGDP
strong
Bos taurus
2.7.1.74
dGTP
-
Bos taurus
2.7.1.74
dTDP
strong
Bos taurus
2.7.1.74
dTTP
-
Bos taurus
2.7.1.74
dUDP
strong
Bos taurus
2.7.1.74
GDP
-
Bos taurus
2.7.1.74
GTP
-
Bos taurus
2.7.1.74
Tris-maleate buffer
-
Bos taurus
2.7.1.74
UDP
strong
Bos taurus
2.7.1.74
UTP
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
kinetic study; linear kinetics with deoxyguanosine or cytidine, bimodal kinetics with deoxycytidine as substrate
Bos taurus
2.7.1.74
1.4
-
cytidine
37°C, pH 8.0
Bos taurus
2.7.1.74
3
-
deoxyguanosine
37°C, pH 8.0
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
-
Bos taurus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Ca2+
activation
Bos taurus
2.7.1.74
Mg2+
below: decrease of activity; maximal activity at a ATP:Mg2+-ratio of 1:1; Mg2+/ATP complex is the reactive species
Bos taurus
2.7.1.74
Mn2+
requirement
Bos taurus
2.7.1.74
Ni2+
activation
Bos taurus
2.7.1.74
Zn2+
activation
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.74
partial
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
thymus
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
0.00523
-
37°C, pH 8.0
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641934
Bos taurus
ADP + 5'-dCMP
-
641934
Bos taurus
?
2.7.1.74
ATP + cytidine
-
641934
Bos taurus
ADP + ?
-
-
-
?
2.7.1.74
CTP + 2'-deoxycytidine
-
641934
Bos taurus
CDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dGTP + 2'-deoxycytidine
best phosphate donor
641934
Bos taurus
dGDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dTTP + 2'-deoxycytidine
-
641934
Bos taurus
dTDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
GTP + 2'-deoxycytidine
-
641934
Bos taurus
GDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
no substrate: dCTP
641934
Bos taurus
NDP + dCMP
-
641934
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
no substrate: guanosine
641934
Bos taurus
NDP + dCMP
-
641934
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
in order of decreasing activity: dGTP, CTP, ATP, dATP, dTTP, UTP, GTP
641934
Bos taurus
NDP + dCMP
-
641934
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
no substrate: adenosine, uridine
641934
Bos taurus
NDP + dCMP
-
641934
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
no substrate: thymidine
641934
Bos taurus
NDP + dCMP
-
641934
Bos taurus
?
2.7.1.74
NTP + deoxyguanosine
-
641934
Bos taurus
NDP + dGMP
-
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
-
641934
Bos taurus
UDP + 2'-deoxy-CMP
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.74
8
-
-
Bos taurus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.74
7
10
about 80% of maximal activity at pH 7 and about 65% of maximal activity at pH 10
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
ADP
-
Bos taurus
2.7.1.74
CDP
strong
Bos taurus
2.7.1.74
CTP
-
Bos taurus
2.7.1.74
cytidine
not
Bos taurus
2.7.1.74
dADP
strong
Bos taurus
2.7.1.74
dATP
strong
Bos taurus
2.7.1.74
dCDP
strong, ATP as substrate
Bos taurus
2.7.1.74
dCMP
ATP as substrate; product inhibition, strong
Bos taurus
2.7.1.74
dCTP
ATP as substrate
Bos taurus
2.7.1.74
deoxycytidine
cytidine as substrate; deoxyguanosine as substrate
Bos taurus
2.7.1.74
deoxyguanosine
not
Bos taurus
2.7.1.74
dGDP
strong
Bos taurus
2.7.1.74
dGTP
-
Bos taurus
2.7.1.74
dTDP
strong
Bos taurus
2.7.1.74
dTTP
-
Bos taurus
2.7.1.74
dUDP
strong
Bos taurus
2.7.1.74
GDP
-
Bos taurus
2.7.1.74
GTP
-
Bos taurus
2.7.1.74
Tris-maleate buffer
-
Bos taurus
2.7.1.74
UDP
strong
Bos taurus
2.7.1.74
UTP
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
kinetic study; linear kinetics with deoxyguanosine or cytidine, bimodal kinetics with deoxycytidine as substrate
Bos taurus
2.7.1.74
1.4
-
cytidine
37°C, pH 8.0
Bos taurus
2.7.1.74
3
-
deoxyguanosine
37°C, pH 8.0
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
-
Bos taurus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Ca2+
activation
Bos taurus
2.7.1.74
Mg2+
below: decrease of activity; maximal activity at a ATP:Mg2+-ratio of 1:1; Mg2+/ATP complex is the reactive species
Bos taurus
2.7.1.74
Mn2+
requirement
Bos taurus
2.7.1.74
Ni2+
activation
Bos taurus
2.7.1.74
Zn2+
activation
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
partial
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
thymus
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
0.00523
-
37°C, pH 8.0
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641934
Bos taurus
ADP + 5'-dCMP
-
641934
Bos taurus
?
2.7.1.74
ATP + cytidine
-
641934
Bos taurus
ADP + ?
-
-
-
?
2.7.1.74
CTP + 2'-deoxycytidine
-
641934
Bos taurus
CDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dGTP + 2'-deoxycytidine
best phosphate donor
641934
Bos taurus
dGDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dTTP + 2'-deoxycytidine
-
641934
Bos taurus
dTDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
GTP + 2'-deoxycytidine
-
641934
Bos taurus
GDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
no substrate: dCTP
641934
Bos taurus
NDP + dCMP
-
641934
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
no substrate: guanosine
641934
Bos taurus
NDP + dCMP
-
641934
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
in order of decreasing activity: dGTP, CTP, ATP, dATP, dTTP, UTP, GTP
641934
Bos taurus
NDP + dCMP
-
641934
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
no substrate: adenosine, uridine
641934
Bos taurus
NDP + dCMP
-
641934
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
no substrate: thymidine
641934
Bos taurus
NDP + dCMP
-
641934
Bos taurus
?
2.7.1.74
NTP + deoxyguanosine
-
641934
Bos taurus
NDP + dGMP
-
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
-
641934
Bos taurus
UDP + 2'-deoxy-CMP
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.74
8
-
-
Bos taurus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.74
7
10
about 80% of maximal activity at pH 7 and about 65% of maximal activity at pH 10
Bos taurus